nm23-H1的表达和突变与卵巢癌浸润转移的关系  

Relationship of Nm23-H1 Expression and Mutation with the Metastasis in Ovarian Carcinoma

作　　者：张洁清[1,2] 李力[1,2] 赵仁峰[1,2] 姚德生 唐步坚[1,2] 何靖育[1,2] 陈心秋 古明华[1,2] 

机构地区：[1]广西医科大学附属肿瘤医院妇科 [2]广西区人民医院妇产科

出　　处：《肿瘤防治研究》1999年第1期9-12,共4页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金

摘　　要：目的：探讨卵巢肿瘤组织中ｎｍ２３－Ｈ１的表达和突变与其浸润转移的关系。方法：采用免疫组织化学，反转录多聚酶链反应（ＲＴ－ＰＣＲ）和多聚酶链反应单链构象多态技术（ＰＣＲＳＳＣＰ）方法检测４１例卵巢癌，２０例卵巢良性肿瘤和２１例正常卵巢组织ｎｍ２３－Ｈ１的表达和突变。结果：（１）卵巢癌组织中ｎｍ２３－Ｈ１阳性表达率明显高于良性卵巢肿瘤及正常对照（Ｐ＜００１）；（２）卵巢癌灶ｎｍ２３－Ｈ１的阳性表达及扩增明显高于转移灶（Ｐ＜００１）；（３）Ⅰ～Ⅱ期患者癌组织中ｎｍ２３－Ｈ１的阳性表达率明显高于Ⅲ～Ⅳ期者（Ｐ＜００１）；（４）ｎｍ２３Ｈ的阳性表达和扩增与病理分级及病理分类无显著差别（Ｐ＞００５）；（５）ｎｍ２３Ｈ１阳性表达的患者近期疗效比阴性者好（Ｐ＜００５）。（６）卵巢癌组织中ｎｍ２３－Ｈ１的突变率为７３％且均发生在晚期患者，而良性肿瘤和正常对照未发现有突变。结论：ｎｍ２３－Ｈ１阳性表达与卵巢癌的浸润转移有关，对它的检测有助于临床上判断预后。Objectives:To observe the amplification and expression of nm23 H1 gene and investigate their clinical significance in ovarian cancer.Methods:Immunohistochemistry,reverse transcription polymerase chain reaction(RT PCR)and single strand conformation polymorphisms(PCR SSCP)procedure were used to detect the mRNA,protein and mutation of nm23 H2 gene in 41 ovarian cancer,20 benign tumors and 21 normal controls.Results:(1) The positive expression of nm23 in ovarian cancer was significantly higher than that in benign tumors and normal controls( P <0 01).(2)The positive expression rate of nm23 H1 gene in primary focus was significantly higher than that in adjacent sites and metastatic lesions( P <0 01).(3) The positive expression rate of nm23 H1 gene in Ⅰ～Ⅱ stages of patient was more higher than that in Ⅲ～Ⅳ stages of patient( P <0 01).(4) No significant correlation was observed between the positive expression rate of nm23 H1 gene and pathological type or grade in ovarian cancer( P >0 05).(5) The short term curative effects in patient with positive expression of nm23 H1 gene was better than that with low expression( P <0 05).(6)The mutation rate of nm23 gene in ovarian carcinoma was 7 3%,but there is no any mutation in benign tumors and normal controls.Conclusion:The expression of nm23 gene is correlated with the metastasis of ovarian carcinoma and may be of value clinically in detecting the metastasis of tumor and in predicting prognosis.

关 键 词：卵巢肿瘤 NM23基因 免疫组织化学 肿瘤转移 

分 类 号：R737.310.2[医药卫生—肿瘤]