DNR荧光直方图诊断白血病细胞耐药的意义  

Implication of Fluorescent Histogram of Daunorubicin (DNR) in Diagnosis of Drug Resistance to Leukemic Cells

作　　者：何明生[1] 陈燕[1] 吴裕丹[1] 李慧玉[1] 喻东姣[1] 

机构地区：[1]同济医科大学附属协和医院血液病学研究所

出　　处：《同济医科大学学报》1999年第1期31-33,共3页Acta Universitatis Medicinae Tongji

基　　金：卫生部科学研究基金

摘　　要：为研究用细胞内柔红霉素（ＤＮＲ）的荧光直方图诊断白血病耐药的方法，用流式细胞仪（ＦＣＭ）检测了２０例急性髓性白血病（ＡＭＬ）的荧光直方图，结合患者的临床耐药表现，耐药细胞的荧光强度诊断界点，耐药指数和荧光指数进行分析。结果，初治４例诊断为药敏，１例诊断为原发耐药；１５例有耐药临床表现的病例，１２例诊断为经典耐药，３例诊断为再生耐药。该方法可快速、简便、直观地在临床上及时发现和评价白血病耐药的类型和程度。To develop a fluorescent histogram of intracellular daunorubicin (DNR) as a diagnostic method for drug resistance in acute myeloid leukemia ( AML) , in combination with drug resistant index(DRI), fluorescent index (FI),a clinically drug resistant manifestation and a cut off point for diagnosis of the fluorescent intensity of drug resistant cells, the histograms of 20 patients with AML were determined and analyzed In 5 patients with de nove AML, 4 cases were diagnosed as having drug sensitivity,and one case as primary drug resistance Among the 15 patients with clinically drug resistant evidance, 12 cases were diagnosed as having classical drug resistance, and 3 cases as regrowth drug resistance Clinically, this method is a rapid,convenient and visual way to find and evaluate a modality and a degree of drug resistance to AML And it may provide a basis for working out chemotherapeutic regimans for each patient

关 键 词：柔红霉素 荧光直方图 白血病 药物疗法 抗药性 

分 类 号：R733.705[医药卫生—肿瘤]