In vivo effects of 17-β-estradiol on plasma immunoglobulin levels and leukocyte density in zebrafish Danio rerio  

作　　者：李磊 张士璀 仝舟 刘佳 

机构地区：[1]Department of Marine Biology, Ocean University of China

出　　处：《Chinese Journal of Oceanology and Limnology》2010年第3期527-532,共6页中国海洋湖沼学报（英文版）

基　　金：Supported by the Natural Science Foundation of Shandong Province (No. 2007ZRB02238);the Natural Science Foundation of Qingdao Municipality (No. 06-2-2-15-jch)

摘　　要：Estradiol, or 17-β-estradiol （E2）, the most potent naturally occurring estrogen, is involved in the hormone-immune system interaction in both mammals and fish. However, in vivo studies are largely limited, and little is known about whether E2 exerts similar effects on both female and male zebrafish （Danio rerio）. Here, we show exposure of both sexes ofD. rerio to 20 nmol/L E2 resulted in a significant increase in Vgl expression, but caused little damage to the hepatocytes, suggesting that this is the optimum E2 concentration. Also, exposure to 20 nmol/L E2 for 20 days caused a marked increase in plasma IgM levels, but had little influence on the peripheral leukocyte density, providing the first evidence of a hormone-immune system interaction in this species.Estradiol, or 17-β-estradiol (E2), the most potent naturally occurring estrogen, is involved in the hormone-immune system interaction in both mammals and fish. However, in vivo studies are largely limited, and little is known about whether E2 exerts similar effects on both female and male zebrafish (Danio rerio). Here, we show exposure of both sexes of D. rerio to 20 nmol/L E2 resulted in a significant increase in Vg1 expression, but caused little damage to the hepatocytes, suggesting that this is the optimum E2 concentration. Also, exposure to 20 nmol/L E2 for 20 days caused a marked increase in plasma IgM levels, but had little influence on the peripheral leukocyte density, providing the first evidence of a hormone-immune system interaction in this species.

关 键 词：ZEBRAFISH Danio rerio 17-Β-ESTRADIOL IMMUNOGLOBULIN leukocytes 

分 类 号：S917.4[农业科学—水产科学]