在AOT/异辛烷反相胶束体系中酶法合成RGD前体二肽  

Enzymatic Synthesis of Precursor Dipeptides of RGD in AOT/Isooctane Reverse Micellar System

作　　者：陈育新[1] 张学忠[1] 陈松明 吴晓霞[1] 

机构地区：[1]吉林大学酶工程实验室

出　　处：《中国生物化学与分子生物学报》1999年第1期148-150,共3页Chinese Journal of Biochemistry and Molecular Biology

摘　　要：近十年来，在有机相中利用酶法合成短肽技术取得了长足的发展．但对于在有机相中合成含有亲水氨基酸的短肽，仍然是一个难题．利用反相胶束可以解决亲水氨基酸在有机相中的低溶解性问题［１］．Ａｒｇ－Ｇｌｙ－Ａｓｐ（ＲＧＤ）是近年来发现的一种具有粘合细胞作用的三肽...The precursor dipeptides of RGD,Boc Arg Gly OEt and Ac Gly Asp diOMe were synthesized in AOT/isooctane reverse micellar system using different proteases.The reaction conditions for the Boc Arg Gly OEt synthesis catalyzed by alcalase and for Ac Gly Asp diOMe synthesis catalyzed by trypsin in AOT/isooctane reverse micelles were optimized by examining the effects of several factors including water content,temperature,pH and reaction time on the dipeptide yields.The best yields for alcalase catalyzed synthesis of Boc Arg Gly OEt and trypsin catalyzed synthesis of Ac Gly Asp diOMe were 62 7% and 78 8%,respectively.

关 键 词：二肽 RGO前体二肽 酶法合成 有机相 

分 类 号：TQ936[化学工程]