erbB-2表达抑制与EGF对p21、p53基因转录活性的促进  

Inhibition of erbB 2 and Promotion Effects of EGF on p21,p53 Gene Transcription in vitro

作　　者：姜琨[1] 高琳[1] 童坦君[1] 

机构地区：[1]北京医科大学生物化学与分子生物学系

出　　处：《中国生物化学与分子生物学报》1999年第1期154-156,共3页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金

摘　　要：原癌基因ｅｒｂＢ－２的异常表达存在于人类多种肿瘤中，与肿瘤的发生、发展密切相关［１］．我们曾构建了反义ｅｒｂＢ－２逆转录病毒重组载体，将其转染存在该基因异常表达的人胃癌细胞系ＢＧＣ－８２３，达到了特异抑制ｅｒｂＢ－２表达、抑制瘤细胞恶性增殖并部分阻断...DNA transfection,Nuclear Run On transcription assay were utilized to investigate the transcription rate of p21,p53 gene under EGF stimulation before and after the significant inhibition of erbB 2 proto oncogene(up to 65%)by its antisense retroviral recombinants.The results showed that after erbB 2 inhibition,EGF stimulation,significantly promoted the transcription of p21(up to 120%)and p53(up to 50%)gene in nuclei from human gastric cancer cells,which demonstrates that the expressional and functional changes of erbB 2 oncogene may be involved in the induction of p21,p53 gene by EGF.

关 键 词：原癌基因 ERBB-2 EGF 转录活性 P21 p53 

分 类 号：R730.2[医药卫生—肿瘤]