溶解氧对细脚拟青霉小试发酵过程及胞内核苷积累的影响  

Effect of controlling dissolved oxygen tension on cell growth and nucleoside production in the mycelium of Paecilomyces tenuipes in a 30L fermentor

作　　者：葛飞[1,2] 黄寅[1] 桂琳[3] 

机构地区：[1]安徽工程科技学院生物化学工程系,安徽芜湖241000 [2]微生物发酵安徽省工程技术研究中心,安徽芜湖241000 [3]皖南医学院微生物和免疫学教研室,安徽芜湖241000

出　　处：《食品工业科技》2010年第7期180-182,186,共4页Science and Technology of Food Industry

基　　金：安徽省高校自然科学基金(KJ2009B071);安徽工程科技学院引进人才科研启动基金(2007YQ005);芜湖市科技计划项目(2008505)共同资助

摘　　要：在30L发酵罐中,考察了溶解氧为20%、40%、60%和80%四个水平对细脚拟青霉发酵过程及胞内核苷积累的影响,结果表明,DO控制在20%、40%、60%和80%时,最大生物量分别在发酵的第13、11、7、5d获得,依次为18·3、21·6、24·7、27·3g/L;最大胞内核苷类物质的产量分别在发酵的第13、13、9、7d获得,依次为3·8643、5·7183、5·2311、4·4968mg/g。在此基础上,提出两阶段DO控制策略,较单阶段DO控制时获得的最大胞内核苷类物质产量和生产率分别提高了19·9%和19·2%。The effect of oxygen supply on cell growth and nucleoside production in the mycelium was studied in a 30L fermentor,with air saturation at 20%,40%,60% and 80%. The results showed that the maximal biomass was 18.3,21.6,24.7,27.3g/L,respectively,at the 13,11,7,5d and maximal nucleoside production was 3.8643,5.7183,5.2311,4.4968mg/g,respectively,at the 13,13,9,7d. Based on the results,a strategy of two stage DO manipulation was proposed. With such a strategy,production and specific formation rate of nucleosides in the mycelium was 19.9% and 19.2% higher than with one stage manipulation.

关 键 词：细脚拟青霉 溶解氧 生物量 核苷 

分 类 号：Q815[生物学—生物工程]