高效液相色谱法测定鸡组织中尼卡巴嗪标识残留物  

HPLC Determination of Nicarbazin Residue in Chicken Tissues

作　　者：谢守新[1] 林海丹[2] 姚仰勋[2] 邵琳智[2] 吴映璇[2] 

机构地区：[1]广州出入境检验检疫局,广东广州510623 [2]广东检验检疫技术中心食品实验室,广东广州510623

出　　处：《食品科学》2010年第10期275-278,共4页Food Science

摘　　要：应用高效液相色谱法测定鸡组织中尼卡巴嗪标识残留物——4,4'-二硝基均二苯脲。试样经乙腈-0.5g/mL氢氧化钾溶液提取,正己烷脱脂净化,用高效液相色谱仪(C18柱)分析,流动相为乙腈-体积分数1%乙酸溶液(53:47,V/V),流速1.0mL/min,紫外检测波长为350nm。对鸡肉、鸡肝和鸡蛋样品进行0.05～0.50mg/kg的药物添加回收实验,4,4'-二硝基均二苯脲的平均回收率为93.6%～102.0%(n=10),相对标准偏差为2.8%～10.4%,方法的定量限为0.05mg/kg。该法简便、准确,适用于鸡组织中尼卡巴嗪标识残留物4,4'-二硝基均二苯脲的检测。A high performance liquid chromatographic （HPLC） assay with UV detection of 4,4＇-dinitrocarbanilide （DNC）, the marker residue for nicarbazin, was developed for the determination of nicarbazin residue in chicken tissues. Samples were extracted with a 50% aqueous potassium hydroxide solution containing acetonitrile, and the resulting extract was then treated with n-hexane for the removal of lipids prior to HPLC analysis using a mobile phase composed of a mixture of acetonitrile and an 1% aqueous acetic acid solution at a flow rate of 1.0 mL/min with UV detection at the 350-nm wavelength. Average recoveries of 10 replicates for DNC in chicken meat, liver and eggs spiked at 3 levels （0.05, 0.20 mg/kg and 0.50 mg/kg） were within the range of 93.6%-102.0%, with a RSD ranging from 2.8% to 10.4%. The method detection limit was 0.05 mg/kg. This method, thanks its simplicity and high accuracy, is most suitable for the determination of nicrabazin residue 4,4＇-dinitrocarbanilide in chicken tissues.

关 键 词：鸡组织 尼卡巴嗪标识残留物 4 4'-二硝基均二苯脲 高效液相色谱法 

分 类 号：O657.72[理学—分析化学]