动物线粒体基因组的转录物作图与应用  

Transcript mapping of mitochondrial genome and its application

作　　者：姚杨[1] 黄原[1] 

机构地区：[1]陕西师范大学生命科学学院,西安710062

出　　处：《生命的化学》2010年第3期434-437,共4页Chemistry of Life

基　　金：国家自然科学基金项目(No.30670279和30970346)资助

摘　　要：线粒体转录物作图是研究线粒体基因组表达、调控和序列注释的基础。目前用于线粒体转录组分析的方法主要有表达序列标签(expressed sequence tag,EST)文库测序、5’和3’cDNA末端快速扩增(rapid amplification of cDNA ends,RACE)技术和RT-PCR方法,采用这些方法已经对果蝇(Drosophila melanogaster)、按蚊(Anopheles funestus)、玻璃海鞘(Ciona intestinalis)、猪(Sus scrofa domestica)等线粒体转录组进行了分析。研究显示,脊椎动物和无脊椎动物的线粒体基因组分别产生3个和5个多顺反子的初级转录物,并通过tRNA间断模型(tRNA punctuation model)加工。线粒体转录物的分析结果为正确注释线粒体基因组序列提供了重要信息。Mitochondrial transcript mapping is a fundamental step to study gene expression,transcriptional regulation,and genome sequence annotation of mitochondrial genome.Several approaches including EST library sequencing,5＇ and 3＇ RACE,and RT-PCR have been used to study mitochondrial transcripts for Drosophila melanogaster,Anopheles funestus,Ciona intestinalis and pig（Sus scrofa domestica）.Many studies show that the mitochondrial genomes from vertebrate and invertebrate produce 3 and 5 primary transcripts,respectively,and these transcripts are processed by tRNA punctuation model.Analysis of the mitochondrial transcript will provide the essential information for mitochondrial genome annotation.

关 键 词：后生动物 线粒体转录物 tRNA间断模型 基因组注释 

分 类 号：Q953[生物学—动物学]