dBcAMP通过上皮生长因子受体间接激活诱导星形胶质细胞形态变化  

dBcAMP Induces Morphological Changes of Asrocytes through Epidermal Growth Factor Receptor

作　　者：李红梅[1] 杨晓临[1] 彭亮[1] 

机构地区：[1]中国医科大学药学院临床药理学教研室,沈阳110001

出　　处：《中国医科大学学报》2010年第6期404-405,409,共3页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30670651);辽宁省教育厅高校科研基金资助项目(2004C055)

摘　　要：目的探讨双丁酰环腺甘酸(dBcAMP)诱导星形胶质细胞形态变化的信号传导通路。方法原代培养星形胶质细胞,采用免疫细胞化学技术检测dBcAMP诱导的星形胶质细胞形态变化,并应用特定抑制剂进一步探讨其形态变化的信号传导机制。结果金属蛋白酶和上皮生长因子受体参与dBcAMP诱导的星形胶质细胞形态变化。结论 dBcAMP诱导的星形胶质细胞形态变化是通过上皮生长因子受体间接激活介导完成的。Objective To study the signaling transduction of dBcAMP induced morphological changes of astrocytes.Methods Morphological changes of primarily cultured mouse astrocytes induced by dBcAMP were studied by immunocytochemical technique.The signaling transduction pathways underlying those changes were further explored using specific inhibitors AG1478 and GM6001.Results Zn2＋-dependent metalloproteinase and epidermal growth factor receptor（EGFR）in astrocytes were involved in the morphological changes of astrocytes induced by dBcAMP.Conclusion dBcAMP-induced transactivation pathways were delineated in the morphological changes of astrocytes.

关 键 词：星形胶质细胞 双丁酰环腺苷酸 间接激活 上皮生长因子受体 

分 类 号：R737.9[医药卫生—肿瘤]