硒杂环化合物SPO清除DPPH和ABTS自由基的光谱学研究  

Spectrometric Investigation of the Antioxidant Activity of a Novel Synthetic Selenadiazole Derivative SPO Against DPPH and ABTS Free Radicals

作　　者：张逸波[1] 郑文杰[2] 黄峙[1] 杨芳[2] 刘杰[2] 陈填烽[2,3] 

机构地区：[1]暨南大学生物系,广东广州510632 [2]暨南大学化学系,广东广州510632 [3]香港中文大学生物系

出　　处：《光谱学与光谱分析》2010年第7期1866-1871,共6页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(20771044);广东省科技计划项目(2007B030703007;2008A030201020);广东省自然科学基金项目(9451063201002077)资助

摘　　要：研究了一种新型硒杂环化合物1,2,5-硒二唑并[3,4-d]嘧啶-5,7-(4H,6H)-二酮(SPO)清除DPPH和ABTS自由基的能力及其光谱学特征。采用分光光度法进行检测,DPPH自由基体系的测定波长为515nm,稳定时间为30 min,而ABTS自由基的测定波长为734 nm,体系在6 min内达到稳定。SPO具有良好的抗氧化活性,能有效、快速地抑制溶液中DPPH和ABTS自由基的形成及其特征吸收峰。在优化的反应体系中,SPO对DPPH和ABTS自由基的半数抑制浓度(IC50)分别为85.2和36.5μmol.L-1,该活性可与标准抗氧化剂Trolox相比拟,且明显优于作为阳性对照的BHA和BHT。实验的结果显示硒杂环化合物在抗氧化方面的广阔应用前景和潜力。The antioxidant activity of a novel synthetic selenadiazole derivative SPO against DPPH and ABTS free radicals was evaluated using spectrometric methods. The results show that the detection wavelength and stable time for DPPH system were 515 nm and 30 min respectively, while those for ABTS system were 734 nm and 6 min, respectively. SPO could effectively and rapidly inhibited the formation of ABTS and DPPH free radicals in a dose- and time-dependent manner, indicating the potent antioxidant activity of SPO under both hydrophilic and hydrophobic conditions. In the optimized systems, the IC50 values of SPO were 85.2 μmol · L^-1 （DPPH assay） and 36.5 μmol · L ^-1 （ABTS assay）, respectively, which were comparable with the standard antioxidant Trolox, and significantly better than the positive controls BHA and BHT. Taken together, our results suggest the potential applications of selenadiazole derivatives as antioxidative agents.

关 键 词：硒 硒杂环化合物 抗氧化活性 DPPH ABTS 

分 类 号：O627.61[理学—有机化学]