心理应激对大鼠肠道敏感性及血浆孤啡肽含量的影响  

Impact of chronic psychological stress on enteric sensitivity and plasma Orphanin FQ level in rats

作　　者：车娅 王鑫 李武良 李萍 

机构地区：[1]西安市第九医院消化科,710054

出　　处：《中国临床研究》2010年第6期446-448,F0002,共4页Chinese Journal of Clinical Research

摘　　要：目的探讨慢性心理应激对大鼠肠道敏感性及血浆孤啡肽(OFQ)含量的影响。方法 80只SD雌性大鼠分为实验组和对照组,实验组大鼠采用束缚应激的方法制作肠易激综合征(IBS)动物模型,并鉴定模型。2组分别于实验0、1、2、3、4周时随机取出8只,用球囊扩张的方法测定肠道敏感性,用放射免疫方法测定血浆OFQ含量。结果 IBS模型经鉴定符合其病理特征,结肠组织学观察显示各组均无明显炎症表现。实验组大鼠各时点肠道敏感性均高于相应对照组(P均<0.01);应激1周时肠道敏感性最高(P均<0.01);应激2周时,肠道敏感性高于3、4周组(P均<0.01);应激3、4周肠道敏感性差异无统计学意义(P>0.05)。实验组大鼠血浆OFQ含量与相应对照组比较均明显升高(P均<0.01),应激1周时最高(P<0.05或P<0.01),2～4周逐渐下降,3、4周时差异无统计学意义(P>0.05),但仍高于应激前水平(P均<0.05)。肠道敏感性与血浆OFQ含量变化趋势一致,二者呈显著正相关(r=0.660,P<0.01)。结论慢性心理应激引起大鼠肠道敏感性及血浆OFQ含量增高;血浆OFQ含量与肠道敏感性变化趋势一致,可能与慢性心理应激大鼠肠道敏感性变化有关。Objective To study the impact of chronic psychological stress on enteric sensitivity and plasma Orphanin FQ（OFQ） level and association between enteric sensitivity and plasma OFQ level in rats.Methods Eighty female Sprague-Dawley rats were randomly divided into experimental group （n=40）and control group（n=40）.Irritable bowel syndrome （IBS） models were established by restrain stress method.The experimental group was further divided into subgroups：0-,1-,2-,3-and 4-week groups according to different time of stress.Enteric sensitivity was detected by balloon dilation method,and plasma OFQ level was detected by radioimmunity method.Results IBS model was reliably constructed by pathological identification.The enteric sensitivity in each subgroup of experimental group were higher than those in corresponding control subgroups （all P0.01）.The enteric sensitivity of 1 week group was the highest in experimental group（all P0.01）,and it was higher in group 2 week than those in group 3 weeks and 4 weeks （all P0.01）,but there already was no significant difference in group 3 weeks and 4 weeks （P0.05）.The plasma OFQ levels were higher in each subgroup of experimental group were higher than those in corresponding control subgroups（all P0.01）.The sensitivity of 1 week group was the highest in experimental group（P0.05 or P0.01）,then gradually decreased in 2-4 weeks groups.It was higher in group 2 week than those in group 3 weeks and 4 weeks （all P0.05）,and there already was no significant difference in group 3 weeks and 4 weeks （P0.05）,but still higher than those before stress （all P0.01）.The plasma OFQ levels had positive correlation with enteric sensitivity（r=0.606,P0.01）.Conclusions Chronic psychological stress may lead to high enteric sensitivity and OFQ level,and significantly positive correlation exists between them,and Therefore the change of enteric sensitivity might associate with plasma OFQ level.

关 键 词：心理应激 易激综合征 肠道敏感性 孤啡肽 

分 类 号：R574.4[医药卫生—消化系统]