卡培他滨对人鼻咽癌细胞CNE-2放射增敏作用的实验研究  

Radiosensitive effect of capecitabine on nasopharyngeal carcinoma cell line CNE-2

作　　者：杨曙[1] 黄晓波[2] 莫凯岚[1] 孙奋勇[3] 

机构地区：[1]广东药学院附属第一医院放疗科,广东广州510080 [2]中山大学肿瘤防治中心放疗科,广东广州510060 [3]暨南大学生物工程研究所,广东广州510632

出　　处：《中国肿瘤外科杂志》2010年第3期156-159,共4页Chinese Journal of Surgical Oncology

摘　　要：目的观察卡培他滨对人鼻咽癌细胞CNE-2的放射增敏作用,并探讨其可能的机制。方法用MTT法测定卡培他滨抑制细胞增殖20%的药物浓度(IC_(20)),以卡培他滨24 h IC_(20)值对CNE-2细胞分别处理3 h、6 h、12 h、24 h,依次设为4个卡培他滨+照射组,对其给予6MeV X线分别单次照射0、2Gy、4Gy、6Gy、8Gy,另设对照组为单纯照射组;用克隆形成试验计算各组的放射增敏比,得到细胞生存曲线;5组细胞均给予6MV X射线单次照射6Gy,流式细胞仪检测各组的细胞周期变化情况及凋亡率。结果卡培他滨的24 h IC_(20)值为0.26μg/mL,卡培他滨处理3 h、6 h、12 h、24 h后的放射增敏比分别为1.08、1.15、1.22和1.35;卡培他滨处理后的照射组可将细胞阻滞在S期,且作用24 h组的细胞凋亡率达45.9%;S期细胞比例及细胞凋亡率与卡培他滨作用时间成正相关。结论卡培他滨(24 h IC_(20))对CNE-2细胞有放射增敏作用,其增敏比随作用时间的延长而增大,24h后增敏作用达最强,主要通过诱导细胞S期阻滞及促进凋亡来影响增敏。Objective To investigate the radiosensitive effect of capecitabine on CNE-2 and its possible mechanism. Methods The non-cytotoxic IC20 was determined by MTT. Non-cytotoxicity was performed by ex- posing CNE-2 cells to capecitabine for 3 h, 6 h, 12 h and 24 h, followed by immediate irradiation （0, 2, 4, 6 and 8 Gy）, the surviving fraction was counted and the sensitization enhancement ratio （SER） was determined by clonogenic assay. Flow cytometry was used to determine cell cycle distribution and apoptosis. Results The IC20 of CNE-2 was 0.26 μg/mL; After exposure to the non-cytotoxic concentration for 3, 6, 12 and 24 h fol- lowed by immediate irradiation （0, 2, 4, 6 and 8Gy） ,the SER was 1.08, 1.15, 1.22 and 1. 35 respective, the apoptotic rate achieved 45.9% ; CNE-2 cells were mainly blocked in S phase after exposure to capecitabine. The proportion of S phase ceils increased along with exposure duration. Conclusions The radiosensitivity en- hancement of capecitabine is accordance with the exposure time, It is significant when radiation is delivered af- ter exposure to capecitabine for 24 h. It is indicated that the radiosensitizing effects could be related to apopto- sis.

关 键 词：卡培他滨 人鼻咽癌细胞系 放射增敏 

分 类 号：R739.63[医药卫生—肿瘤]