FAP在胃癌间质中的表达及其与微血管密度的关系  

A study on FAP expression and correlation with MVD in the stroma of gastric carcinoma

作　　者：邵洪江[1] 靳占峰[1] 

机构地区：[1]哈尔滨医科大学基础医学院法医教研室,150081

出　　处：《国际遗传学杂志》2010年第3期134-137,146,共5页International Journal of Genetics

基　　金：基金项目：黑龙江省政府博士后资助基金（LRB07-114）;哈尔滨医科大学基础医学院留学归国科研启动基金

摘　　要：目的探讨FAP在胃癌间质中的表达及其与微血管密度（microvesseldensity，MVD）的关系。方法收集162例胃癌组织，同时建立人胃癌细胞系SGC-7901裸鼠皮下移植瘤模型，采用免疫组织化学染色法检测FAP和CD34的表达。结果在人胃癌组织中，FAP表达于间质成纤维细胞，表达阳性率为90．74％（t47例），而胃癌细胞和正常胃组织中无表达，且FAP表达和MVD分别与胃癌的分化程度（x2=51．882，P〈0．01；x2=46．383，P〈0．01）、侵袭深度（x2=40．193，P〈0．01；x2=21．617，P〈0．01）及淋巴结转移（r=24．232，P〈0．01;x2=13．393，P〈0．01）有关。在15例裸鼠皮下移植瘤中，所有肿瘤中FAP均不同程度在间质成纤维细胞呈阳性表达。在人胃癌组织（x2=97．710，P〈0．01）和裸鼠皮下移植瘤组织（x2=11．100，P〈0．01）中，不同强度的FAP表达组间肿瘤间质MVD的差异具有统计学意义，且随着FAP表达水平的增高，MVD也随之增加。结论FAP的表达与胃癌的分化程度、侵袭深度及淋巴结转移有关。FAP可能通过促进肿瘤的微血管生成，加快肿瘤的生长、侵袭和转移。Objective To investigate the expression of fibroblast activation protein （FAP） in human gastric carcinoma（GC） and the relationship between FAP expression and microvessel density （MVD）. Methods Human GC specimens from one hundred sixty-two patients were available for study. Nude mice model was subcutaneously transplanted with human gastric carcinoma SGC-7901 cells. The expression of FAP and CD34 in gastric carcinoma tissue was detected by immunohistochemistry. Results FAP expresses in stromal fibroblasts in 90.74% （147 cases） patients with GC, while carcinoma cells and normal gastric tissues are negatively for FAP. In gastric carcinoma, FAP expression and MVD associate with tumor invasion （x2=51. 882,P〈0.01;x2= 46. 383, P 〈 0.01 ）, differentiation （X2 = 40. 193, P 〈 0. 01 ; X2 = 21. 617, P 〈 0.01 ） and lymph node metastasis（x2 =24. 232,P 〈0. 01 ;X2 = 13. 393,P 〈0. 01 ）. In fifteen cases of tumor tissue from nude mice transplanted subcutaneously with human gastric carcinoma SGC-7901 cells, all tumors show different degrees of FAP expression. MVD significantly correlates with FAP intensity in both human gastric carcinoma（x2 = 97. 710 ,P 〈0.01 ） and mouse tumor tissue（x2 = 11. 100,P 〈0. 01 ）. MVD increases in samples with increased FAP expression. Conclusion In GC, FAP expression correlates with the invasion and metastasis. FAP may promote tumor growth, invasion and metastasis by facilitating tumor angiogenesis.

关 键 词：胃癌 成纤维细胞激活蛋白 肿瘤血管生成 SGC-7901细胞系 裸鼠 

分 类 号：R735[医药卫生—肿瘤]