汉族人群人类血小板抗原-1系统a/b/x等位基因多态性调查  

Distribution of HPA-1a/b/x allele polymorphism in Chinese Hans

作　　者：沈彤[1] 冯明亮[1] 印彪[2] 赵玉林[2] 刘熔增[2] 刘达庄[1] 

机构地区：[1]上海市血液中心,上海200051 [2]华东师范大学

出　　处：《临床血液学杂志（输血与检验）》2010年第3期321-323,共3页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

基　　金：上海市自然科学基金资助课题(No:05ZR14107)

摘　　要：目的:调查HPA-1抗原系统a/b/x等位基因在汉族人群中的多态性分布。方法:利用聚合酶链反应-序列特异引物技术(PCR-SSP)技术对1000例中国无关健康汉族人群样本进行HPA-1抗原系统等位基因分型,随机抽取部分样本进行测序验证。结果:本次调查共检测到HPA-1a/a基因型988个,HPA-1a/b基因型12个,未检测到HPA-1x等位基因。结论:HPA-1抗原系统在中国汉族人群中以HPA-1a等位基因为主(99.4%),HPA-1b分布频率较低(0.6%)。目前国内尚未发现HPA-1x等位基因,有必要进一步扩大样本对不同地区、不同民族进行多态性调查。Objective：To investigate the polymorphism of HPA-1 antigen system a/b/x alleles in Chinese Han population.Method：HPA-1 antigen system alleles of 1000 samples from unrelated healthy Chinese Han population were genotyped by PCR-SSP（Polymerase Chain Reaction-Sequence Specific Primed）,part of samples were selected randomly and sequenced for confirmation.Result：988 genotypes of a/a and 12 of a/b alleles in HPA-1 antigen system were identified,none of HPA-1x allele were found.Conclusion：Of the identified HPA-1 antigen system polymorphisms,HPA-1a allele is primary in Chinese Han population（the frequency is 99.4%）,the frequency of HPA-1b polymorphism distribution is low（0.6%）.In the present study,none of HPA-1x alleles have identified in China,further investigation on polymorphism distribution in various areas and populations is needed with larger samples.

关 键 词：中国汉族人群 HPA-1 等位基因 多态性 

分 类 号：Q343.1[生物学—遗传学]