阿托伐他汀对大鼠局灶性脑缺血后胡须体觉皮层功能的影响及其机制  

Effects and related mechanisms of atorvastatin on functions of whisker barrel cortex in rats exposed to focal cerebral ischemia

作　　者：张云云[1] 姜婧[1] 丁新生[1] 李文磊[2] 孙波[1] 黄素素[1] 解渊[1] 梁汝庆[1] 

机构地区：[1]南京医科大学第一附属医院神经内科,江苏南京210029 [2]江苏省中医院神经内科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2010年第6期776-780,836,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助(309710221H0906);江苏省自然科学基金资助(BK20084710)

摘　　要：目的:探讨不同剂量的阿托伐他汀对大鼠局灶性脑缺血后胡须体觉皮层功能的影响及其机制。方法:30只大鼠训练达标后依随机数字表法分为假手术组、对照组(0.1ml/kg生理盐水)、低剂量组(1.0mg/kg)、中剂量组(3.0mg/kg)、高剂量组(6.0mg/kg)。对照组及各剂量组采用显微镜下结扎大脑中动脉数根分支构建局灶性胡须体觉皮层缺血模型并分别于造模后24h开始灌胃,每天1次,连续7天。造模后每天进行胡须依赖实验检测直至达到标准并于术后14天,应用超声多普勒检测缺血区周围的血流量;应用免疫组化检测缺血区周围CD34及VEGF的表达情况。结果:与对照组相比,低、中、高剂量组均增强了胡须依赖辨别能力(P<0.01,P<0.01,P<0.05);同时提高了缺血区周围的血流量(P<0.01,P<0.01,P<0.05);增加了缺血区周围CD34、VEGF阳性细胞数(P<0.01,P<0.01,P<0.05)。而且中剂量组与低剂量、高剂量组相比,差异有统计学意义(P<0.05,P<0.01)。结论:阿托伐他汀有助于缺血后胡须依赖辨别能力的改善,其机制可能通过促进VEGF的表达及增加局部的微血管密度从而提高局部血流,且以中剂量效果较好。Objective：To study the effects and related mechanisms of atorvastatin on functions of whisker barrel cortex in rats exposed to focal cerebral ischemia. Methods：Thirty male Sprague-Dawley rats,which had been trained to reach criteria,were randomly divided into sham-operation group,control group,low-dose group,middle-dose group and high-dose group. The focal ischemia model of whisker-barrel cortex was established by ligating 2-3 branches of right middle cerebral artery permanently. Animals of each dose and control group were administered with drug and saline 24h after the operation. The functions of whisker barrel cortex were evaluated each day after ischemia until rat reached the criteria again. Local cerebral blood flow was assessed by laser Doppler scanner and the expression of VEGF and CD34 was detected with immunohistochemistry method 14d after operation. Results：Compared with the control group,the functions of whisker barrel cortex were significantly improved in each dose group（P 〈 0.01 ,P 〈 0.01 ,P 〈 0.05） ;the local cerebral blood flow and the numbles of VEGF, CD34 positive cells were increased in animalsreceining differene-dose atorastatin than that of control group （P 〈 0.01 ,P 〈 0.01 ,P 〈 0.05 respectirely）. Middle-dose atorvastatin was the best for function improvement of whisker barrel cortex in rats （P 〈 0.05,P 〈 0.01）. Conclusion：Atovastatin improved the functions of whisker barrel cortex,which may be related to its increase of the numbles of VEGF positive cells and microvascular density and enhancement of local cerebral blood flow.

关 键 词：阿托伐他汀 胡须体觉皮层 脑缺血 VEGF 

分 类 号：R743[医药卫生—神经病学与精神病学]