siRNA沉默Annexin-1基因对膀胱癌T24细胞顺铂耐药的影响  

The changes of DDP sensitivity after annexin-1 silencing by small interfering RNA of bladder cancer T24 cells

作　　者：高琳[1] 杨远航 徐万海[1] 林相国[1] 李建章[1] 杨德君[1] 王晓民[1] 

机构地区：[1]哈尔滨医科大学附属第四医院泌尿外科,黑龙江哈尔滨150001 [2]澳大利亚Month大学

出　　处：《中国老年学杂志》2010年第12期1680-1683,共4页Chinese Journal of Gerontology

基　　金：黑龙江省科技攻关(国际合作)项目(编号:WB07C06)

摘　　要：目的观察RNA干扰沉默膜联蛋白-1(Annexin-1)基因表达技术对膀胱癌T24细胞Annexin-1基因表达和顺铂耐药的影响。方法针对Annexin-1基因不同部位设计并化学合成3对不同的靶向小分子干扰RNA(siRNA),脂质体介导瞬时转染膀胱癌T24细胞,转染后应用半定量RT-PCR和Western印迹检测Annexin-1mRNA和蛋白的改变,用MTT法检测沉默Annexin-1表达在膀胱癌T24细胞对顺铂敏感性的变化。结果转染siRNA后膀胱癌T24细胞Annexin-1的mRNA和蛋白水平显著下降(P<0.05),增强细胞对顺铂的敏感性(P<0.05)。结论 RNA干扰Annexin-1基因后其mRNA和蛋白水平显著下降,并且增强T24细胞对顺铂的敏感性。Objective To explore the effects of annexin-1 gene silencing by small interfering RNA （siRNA） on bladder cancer cells T24 and the sensitivity change of DDP. Methods Three siRNA targeting three different domains of annexin-1 gene were designed, synthesized, and transfected into bladder cancer T24 cells by lipofectamineTM2000. After transfection, expression of annexin-1 was detected by RT-PCR and West-blotting. The cellular sensitivity to DDP was evaluated by MTT assay. Results Compared with the negative control （ siR- NA-NC） groups, transfection of three targeting siRNA in 3＂24 cells decreased the expression of annexin-1 mRNA and protein. Annexin-1 gene silencing increased the sensitivity of 3＂24 cells to DDP. Conclusions The chemically synthesized specific siRNA targeting annexin-1 could effectively reduce the expression of annexin-1 gene, increase the sensitivity of T24 cells to DDP.

关 键 词：膀胱癌 膜联蛋白-1 SIRNA 顺铂 

分 类 号：R737.14[医药卫生—肿瘤]