Complete genome sequence and proteomic analysis of a thermophilic bacteriophage BV1  

作　　者：LIU Bin WU Suijie XIE Lianhui 

机构地区：[1]Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China [2]College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China [3]Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China

出　　处：《Acta Oceanologica Sinica》2010年第3期84-89,共6页海洋学报（英文版）

基　　金：The Key Natural Science Foundation of Fujian under contract No. 2007J0004;the National Natural Science Foundation of China under contract No. 40576076

摘　　要：Viruses of thermophiles are of great interest due to their roles in gene transfer, global geochemical cycle and evolution of life on earth. However, the thermophilic bacteriophages have not been studied extensively. In this investigation, a typical bacteriophage BV1 was obtained from a thermophilic bacterium Geobacillus sp. 6k512, which was isolated from an inshore hot spring in Xiamen of China. The BV1 contained a double-stranded linear DNA of 35 055 bp, which encodes 54 open reading frames （ORFs）. Interestingly, eight of the 54 BV1 ORFs shared sequence similarities to genes from human disease-relevant bacteria. Seven proteins of the purified BV1 virions were identified by proteomic analysis. Determination of BV1 functional genomics would facilitate the better understanding of the mechanism for virus-thermophile interaction.Viruses of thermophiles are of great interest due to their roles in gene transfer, global geochemical cycle and evolution of life on earth. However, the thermophilic bacteriophages have not been studied extensively. In this investigation, a typical bacteriophage BV1 was obtained from a thermophilic bacterium Geobacillus sp. 6k512, which was isolated from an inshore hot spring in Xiamen of China. The BV1 contained a double-stranded linear DNA of 35 055 bp, which encodes 54 open reading frames （ORFs）. Interestingly, eight of the 54 BV1 ORFs shared sequence similarities to genes from human disease-relevant bacteria. Seven proteins of the purified BV1 virions were identified by proteomic analysis. Determination of BV1 functional genomics would facilitate the better understanding of the mechanism for virus-thermophile interaction.

关 键 词：THERMOPHILE BACTERIOPHAGE genome sequence proteomic analysis 

分 类 号：Q987[生物学—遗传学] S512.101[生物学—人类学]