含尿素酶基因的重组变链菌JH1140变链素对变链菌UA159的抑制作用  

The inhibition effect of recombinant Streptococcus mutans JH1140 containing urease gene on Streptococcus mutans UA159

作　　者：芮昕[1] 冯希平 

机构地区：[1]上海交通大学医学院附属第九人民医院·口腔医学院口腔预防科,上海市口腔医学重点实验室,上海200011

出　　处：《上海口腔医学》2010年第3期281-284,共4页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30572038);上海市科学技术委员会科研项目(05JC14042,08DZ2271100);上海市重点学科建设项目(S30206)

摘　　要：目的:检测重组变形链球菌JH1140的变链素抑制其他变形链球菌(变形链球菌UA159)的效果。方法:以变形链球菌UA159为指示菌,选取野生型变形链球菌JH1140和重组变形链球菌JH1140(各20例)与变形链球菌UA159共同培养,观察记录抑菌环直径。采用SAS9.1软件包进行方差分析,比较2种细菌的抑菌环直径,分析抑菌能力。以不同浓度变形链球菌UA159为指示菌,取野生型变形链球菌JH1140和重组变形链球菌JH1140(各20例)与变形链球菌UA159共同培养,观察记录抑菌环直径。采用SAS9.1软件包进行方差分析,比较不同浓度指示菌条件下,重组变形链球菌JH1140的抑菌环直径。结果:相同指示菌浓度下,野生型变形链球菌与重组变形链球菌的抑菌环直径之间未见显著差别,P>0.05。不同浓度指示菌条件下,重组变形链球菌JH1140的抑菌环直径未见显著差别,P>0.05。结论:重组重组变形链球菌JH1140与野生型变形链球菌JH1140相比,抑菌能力未见改变。重组细菌在表达新基因的同时,其变链素分泌未受影响。PURPOSE： To study the effect of mutacin of recombinant Streptococcus mutans JH1140 against Streptococcus mutans UA159. METHODS： Wild Streptococcus mutans JH1140 （20 samples） and recombinant Streptococcus mutans JH1140 （20 samples） were respectively co-cultured with Streptococcus mutans UA159 for 24 hours.One-way t test was used via SAS9.1 software package to determine the statistical difference of the inhibitory rings of the two groups.Four different concentrations of indicative strain Streptococcus mutans UA159 were used in the inhibition with the original Streptococcus mutans JH1140（20 samples） and the recombinant Streptococcus mutans JH1140（20 samples）.The diameters of the inhibitory rings were analyzed with ANOVA via SAS 9.1 software package among the eight groups. RESULTS： The diameters of the inhibitory rings between the wild Streptococcus mutans JH1140 and recombinant Streptococcus mutans JH1140 were not statistically significant （P0.05）.The diameters of the inhibitory rings among the four groups that the recombinant Streptococcus mutans JH1140 acted against the fours different indicative strains were not statistically significant （P0.05）. CONCLUSIONS： Compared with the wild Streptococcus mutans JH1140 ,the mutacin inhibitory ability of the recombinant Streptococcus mutans JH1140 nearly has no change.And the concentration of indication stains can not affect the mutacin inhibitory abilities of the recombinant Streptococcus mutans JH1140.Supported by National Natural Science Foundation of China （Grant No.30572038）,Research Fund of Science and Technology Commission ofShanghai Municipality （Grant No.STCSM-05JC14042,08DZ2271100） and Shanghai Leading Academic Discipline Project （Grant No.S30206）.

关 键 词：变形链球菌 变链素 基因重组 尿素酶 

分 类 号：R781.1[医药卫生—口腔医学]