黄牛瘤胃中多不饱和脂肪酸氢化的影响因素  

Influencing factors of polyunsaturated fatty acids(PUFA) hydrogenation in cattle rumen

作　　者：陈艳乐[1] 叶子弘[2] 蒋林树[3] 

机构地区：[1]温州大学瓯江学院,浙江温州325035 [2]中国计量学院生命科学学院,浙江杭州310018 [3]北京农学院动物科技系,北京102206

出　　处：《浙江农业学报》2010年第3期292-297,共6页Acta Agriculturae Zhejiangensis

基　　金：温州市科技计划项目(N20080018)

摘　　要：为探索黄牛瘤胃中影响多不饱和脂肪酸氢化的因素,通过体外模拟瘤胃发酵的方法,研究不同补充料、瘤胃微生物、培养时间对瘤胃氢化作用的影响。结果表明:微生物培养时间所引起的变异占NH3-N总变异的64.20%,是引起NH3-N变化的主要影响因素;不同补充料、微生物区系和培养时间对挥发性脂肪酸(VFA)各组分的影响有显著差异;微生物培养时间与微生物区系的互作对丙酸、异丁酸和丁酸具有显著影响(V微生物×时间分别为43.32**,1.56**和2.87**);乙酸、异戊酸、戊酸不受微生物区系、培养时间、不同补充料的影响;以膨化大豆为补充料时,NH3-N的含量最高(19.49mg/mL)且显著高于焙烤大豆;不同的原料和培养时间及其二者间的相互作用将对硬脂酸含量产生显著的影响(三者引起的变异占硬脂酸总变异的77.76%),而瘤胃微生物区系只在特定的培养时间才对硬脂酸含量产生显著影响;亚油酸(C18∶2)浓度只受到不同的瘤胃微生物的影响,不同的原料、培养时间和瘤胃微生物区系间的互作对反式油酸(C18∶1)浓度、亚麻酸(C18∶3)浓度均有显著或极显著的影响。The method of rumen fermentation in vitro was used to study the effect of various supplementary feeds,rumen micro-organisms,incubation time on cattle rumen hydrogenation.The results showed that 64.20% variation of NH3-N was affected by incubation time,which was the main effect of variation of NH3-N;various concentrate supplement,rumen micro-organisms and incubation time had significantly different effects on various VFA components;the interaction between incubation time and rumen micro-organisms affected propanoic acid,isobutyric acid,butyric acid significantly（variances of interaction effect were 43.32＊＊,1.56＊＊ and 2.87＊＊,respectively）.Acetic acid,isovaleric acid and valeric acid were free from the impaction of rumen micro-organisms,incubation time and different supplement.The highest concentration of NH3-N（19.49 mg·mL-1） was observed when extruded soybean was used as supplement.Different supplement,incubation time and interaction between them significantly affected the concentration of stearic acid（totally 77.76% variation was attributed to these three components）.The remarkable influence on the content of stearic acid was produced only at specific time in rumen microflora.Concentration of linoleic acid（18∶2） was impacted only by different rumen microflora.Different material,incubation time and interaction in rumen microflora significantly affected the concentration of trans-oleic acid（18∶1） and linolenic acid（18∶3）.

关 键 词：补充料 培养时间 微生物区系 氢化作用 多不饱和脂肪酸 

分 类 号：S852.23[农业科学—基础兽医学]