2型糖尿病患者结膜上皮细胞凋亡及炎症状态分析  

The apoptosis and expression of TGF-β1 and NF-κB of conjunctival epithelium cell in diabetes mellitus patients

作　　者：卢迪[1] 金龙山[1] 李文吉[1] 崔仁哲[1] 孟欢[1] 

机构地区：[1]延边大学附属医院眼科,吉林延吉133000

出　　处：《中国实用眼科杂志》2010年第6期591-593,共3页Chinese Journal of Practical Ophthalmology

摘　　要：目的 了解2型糖尿病患者结膜上皮细胞凋亡和炎症状态及其与2型糖尿病患者易患干眼的关系.方法 随机选择2型DM患者35例（35只眼）为实验组、正常人25名（25只眼）为对照组.所有受试者均接受基础泪液分泌试验（SIt）、泪膜破裂时间（BUT）及角膜荧光染色（FL）检查,采用印迹细胞法获得结膜上皮细胞,Annexin v-FITC/PI流式细胞技术（FCM）定量检测细胞凋亡;并用免疫印迹细胞化学染色法检测结膜上皮细胞转化生长因子-β1（TGF-β1）及核转录因子-κB（NF-κB）的表达.结果 2型DM组BUT、SIt值较对照组明显减少（P〈0.01）;2型DM组FL较对照组明显增强（P〈0.01）;2型DM组结膜上皮细胞凋亡指数较对照组明显增强（P〈0.01）;TGF-β1和NF-κB表达较对照组明显增强（P〈0.05）;2型DM组结膜上皮细胞凋亡指数与TGF-β1和NF-κB的表达呈正相关（P〈0.01）;2型DM组BUT、SIt值与结膜上皮细胞凋亡指数及TGF-β1和NF-κB的表达呈负相关（P〈0.01）;2型DM组FL与结膜上皮细胞凋亡指数及TGF-β1和NF-κB的表达呈正相关（P〈0.01）. 结论2型DM患者结膜上皮细胞凋亡和炎症明显增强,这可能是2型DM患者易患干眼的重要原因之一.Objective To apprehend the ocular surface condition of type 2 diabetes mellitus patients by detecting the apoptosis index and the inflammation condition of conjunctival epithelial cell. To analyze the reason of the type 2 DM＇ s dry eye. Methods Thirty-five type 2 DM patients and 25 healthy adults were se lected randomly to detect the break-up time （BUT ）, Schirmer I test （SIt） and Fluorescein staining （FL）. Con junctival epithelial cells were collected by impression cytology from the type 2 DM patients and healthy adults. Flow cytometry was taken to measure apoptosis. Immunohistochemical staining was performed to detect trans forming TGF-P ,and NF-K B. Results The value of SIt and BUT in type 2 DM patients obviously increased man those in control group （P 〈0.01）. The FL in type 2 DM patients positively expressed than that in control group （P〈0.01）. The apoptotic index of conjunctival epithelium in type 2 DM patients was significantly higher than that in control group （P 〈0.01）. The expression of TGF-β1 ,and NF-k B in DM patients was obviously stronger than those in control group （P 〈0.01）. The apoptotic index showed positive correlation with the ex pression of TGF-＊ 1 （P〈0.01）. Conclusions The increase of apoptotic and inflammation of conjunctival ep ithelium may have close relationship with the type 2 DM＇ s dry eye.

关 键 词：2型糖尿病 结膜上皮细胞 转化生长因子-Β1 核转录因子-ΚB 凋亡 干眼 

分 类 号：R779.6[医药卫生—眼科]