雷帕霉素联合顺铂对卵巢癌SKOV-3细胞增殖的抑制作用  

Rapamycin combined with cisplatin in inhibiting the proliferation of ovarian cancer SKOV-3 cells

作　　者：段钊[1] 杨雪梅 邢兰瑛[1] 潘艳艳[1] 王艳华[1] 李虹[1] 

机构地区：[1]西安交通大学医学院第二附属医院妇产科,陕西西安710004 [2]陕西省肿瘤医院妇瘤科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2010年第4期493-496,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的探讨雷帕霉素单独及联合顺铂应用对人卵巢癌SKOV-3细胞生长的影响及可能的分子机制。方法体外培养人卵巢癌SKOV-3细胞,实验组分别采用不同浓度的雷帕霉素、顺铂及40nmol/L雷帕霉素+10μmol/L顺铂作用,MTT法检测上述药物对细胞的增殖抑制率;流式细胞仪检测分析细胞凋亡率及细胞周期分布变化。结果雷帕霉素单独及联合顺铂应用均可显著抑制人卵巢癌SKOV-3细胞的增殖,其作用呈现时间-剂量依赖性,且两药联合作用时抑制率显著增高(P<0.05),显示两药有协同作用;流式细胞仪检测显示雷帕霉素能使卵巢癌SKOV-3发生G1期阻滞,同时可诱导细胞凋亡,其凋亡率随用药浓度增加而增加。结论雷帕霉素和顺铂可显著地抑制人卵巢癌SKOV-3细胞的增殖,阻滞细胞周期并诱导细胞凋亡,且两药联合应用时呈现协同作用。Objective To study rapamycin alone or in combination with cisplatin on the growth of human ovarian cancer SKOV-3 cells and the possible molecular mechanism. Methods Human ovarian cancer SKOV-3 cells were cultured in vitro. The experimental group was administered with different concentrations of rapamycin and cisplatin,and 40 nmol/L rapamycin＋10 μmol/L cisplatin. MTT assay was applied to examine these drugs＇ inhibition rate on cell proliferation; flow cytometry was used to analyze the apoptosis rate and cell cycle distribution. Results Rapamycin alone or in combination with cisplatin could significantly inhibit the proliferation of human ovarian cancer SKOV-3 cells in time-and dose-dependent manners,and the joint effect of the two drugs had a significantly higher inhibition rate （P0.05）,showing their synergistic effects. Flow cytometry revealed that rapamycin could cause G1 phase arrest of ovarian cancer SKOV-3 cells and induce apoptosis at the same time; the apoptosis rate increased with the increase in drug concentration. Conclusion Rapamycin and cisplatin can significantly inhibit the proliferation of human ovarian cancer SKOV-3 cells,arrest cell cycle and induce cell apoptosis,and the two drugs administered in combination show synergistic effects.

关 键 词：雷帕霉素 顺铂 卵巢癌SKOV-3细胞 细胞周期 细胞凋亡 

分 类 号：R737.31[医药卫生—肿瘤]