气态甲醛致小鼠骨髓细胞DNA-蛋白质交联的研究  

Gaseous Formaldehyde-Induced DNA-Protein Crosslinks in Mouse Bone Marrow Cells

作　　者：程文文[1] 李兰[1] 胡传禄[1] 魏晨曦[1] 杨旭[1] 丁书茂[1] 

机构地区：[1]华中师范大学遗传调控与整合生物学湖北省重点实验室,武汉430079

出　　处：《生态毒理学报》2010年第2期262-267,共6页Asian Journal of Ecotoxicology

基　　金：国家科技支撑计划项目(No.2006BAJ02A10)

摘　　要：为探讨吸入性甲醛能否对小鼠骨髓造血细胞产生遗传毒性,以SPF级昆明雄性小鼠为材料,采用动态吸入方式连续染毒72h,取骨髓细胞,测定DNA-蛋白质交联.结果发现,随着甲醛浓度的升高(0、0.5、1.0、3.0mg·m-3),小鼠骨髓细胞DNA-蛋白质交联系数逐渐升高,0.5mg·m-3组与对照组存在显著差异(p<0.05),1.0、3.0mg·m-3组与对照组存在极显著差异(p<0.01).表明,在实验浓度范围内,甲醛对小鼠骨髓细胞具有一定的遗传毒性.In order to study the genotoxicity of gaseous formaldehyde（FA）on mouse bone marrow cells,SPF level Kunming male mice were exposed to gaseous formaldehyde for 72h.KCl-SDS assay were applied to detect DNA-protein crosslinks（DPC）in bone marrow cells.Results showed that low concentration（0.5mg·m-3）gaseous formaldehyde could induce DPC formation significantly（compared with the control,p0.05）,and high concentrations（1.0,3.0mg·m-3）could induce DPC formation very significantly（p 0.01）.DPC was increased with the increasing of gaseous formaldehyde concentrations（0.5,1.0,3.0mg·m-3）.The results suggested that among the concentrations investigated in the present study,gaseous formaldehyde had obvious genetic toxicity on the mouse bone marrow cells.

关 键 词：甲醛 骨髓细胞 DNA-蛋白质交联 

分 类 号：X131[环境科学与工程—环境科学] X171.5