酵母异源功能互补在植物基因克隆中的应用  

Application of Functional Complementation of Yeast Mutants in Cloning Plant Genes

作　　者：张怡[1] 李成伟[1] 

机构地区：[1]周口师范学院生命科学系,周口466001

出　　处：《生物技术通报》2010年第7期14-21,共8页Biotechnology Bulletin

基　　金：转基因生物新品种培养重大专项子任务(2008ZX08010-004)

摘　　要：酵母作为研究高等真核生物的一种重要的模式生物,不仅在生物信息学方面发挥着重要的作用,其丰富的突变体在其他生物基因克隆和功能验证等方面也发挥着重要的作用。酵母异源互补方法是利用酵母突变体验证相应性状异源基因功能,或通过文库筛选克隆相应性状异源基因,为高等真核生物的基因克隆和功能验证提供了一种捷径。主要对酵母异源功能互补法在研究植物基因方面的进展做一概述。As the important model organism for researching higher eukaryotic organisms,yeast not only plays a significant role in bioinformatics,its abundant mutants are also employed in gene cloning and function identification of other organisms. The method of functional cemplementation of yeast mutant strains can be used to verify the function of heterologus genes from other organisms,or to clone genes with corresponding traits by using cDNA library,which provides a shortcut for gene cloning and function identification of other eukaryotic organisms. In this review,we briefly summarized the research progress on functional complementation of yeast mutants in studying plant genes.

关 键 词：酵母 生物信息 酵母异源功能互补 基因克隆 功能验证 

分 类 号：Q943.2[生物学—植物学]