凝血酶调节蛋白基因预防动脉损伤-阻滞模型血栓形成的实验研究  

The Experimental Study on Preventive Effects of hTM Gene for Thrombogenesis in Rabbit Artery Injury Model

作　　者：戴毅[1] 黄晓玲[2] 孟敏[1] 李敬东[1] 时德[3] 

机构地区：[1]川北医学院附属医院普外科,南充637000 [2]重庆医科大学第一附属医院超声科,重庆400014 [3]重庆医科大学第一附属医院血管外科,重庆400014

出　　处：《血栓与止血学》2010年第3期106-109,共4页Chinese Journal of Thrombosis and Hemostasis

摘　　要：目的观察pcDNA3.1/hTM质粒转染表达的兔动脉损伤模型中的血栓形成情况。方法用注射式加压转染的方式对兔动脉壁转染pcDNA3.1/hTM质粒,再制造动脉损伤-阻滞模型,于术后3d、7d用彩色多普勒观察活体吻合口内径和血流流速;再做病理切片H-E染色,计算血管血栓形成面积。结果彩色多普勒检查证实,实验组动脉吻合口光滑,血流平稳;而空载质粒转染组和对照组动脉吻合口明显狭窄,血流紊乱,有"花色"血流。3d、7d时间点各组血栓面积分别为35%±15%、33%±17%(实验组);66%±26%、74%±21%(空载质粒转染组);62%±22%、73%±24%(对照组)。结论凝血酶调节蛋白(thrombomodulin,TM)基因可以明显减少动脉损伤-阻滞模型的血栓形成。Objective To observe thrombogenesis in rabbit artery injury-block model which were transferred by pcDNA 3.1/h TM plasmids.Methods After transferred pcDNA 3.1/h TM plasmid into rabbit artery by high-pressure injection,rabbit common iliac artery were cut and anastomosed again.At the 3 days and 7days after second operation,we checked inside diameter of anastomotic stoma and blood flow velocity by color Doppler.The treated artery were sliced and stained by H-E.Local thrombus formation was calculated by computer.Results Color Doppler showed that the anastomotic stoma was smooth and the blood flow velocity was steady at hTM treated group.In contrast,the anastomotic stoma was stenosis at the vector control group and buffer control group.＂mosaic＂ flow could be see in artery lumen at the two control group.Conclusion Human thrombmodulin gene could reduce thrombogenesis in rabbit artery injury-block model.

关 键 词：凝血酶调节蛋白 基因 抗血栓 动物模型 

分 类 号：R364.1[医药卫生—病理学]