黑木耳漆酶酶学性质分析  

Analysis on Laccase Properties of Auricularia auricular

作　　者：张利明[1] 高玉千[1] 贾海军[1] 蒋瑾[1] 张庭静[1] 徐淑霞[1] 张世敏[1] 吴坤[1] 

机构地区：[1]河南农业大学生命科学学院,河南郑州450002

出　　处：《山东农业科学》2010年第6期66-68,共3页Shandong Agricultural Sciences

基　　金：河南省科技厅重点攻关项目"产漆酶工程菌的构建及高效表达"(82102220011)

摘　　要：从12个黑木耳菌株中筛选出一株黑木耳漆酶高产菌株——地茂1号,对其进行Native-PAGE,发现其含有3种漆酶同工酶。对其发酵液进行酶学性质分析,发现地茂1号在液体培养基中第11天酶活达到最大,为382.6 U/L,最适温度为50℃,最适pH值为3.2,温度及pH稳定性较好。Dimao 1 with high production of laccase was screened from twelve strains of Auricularia auricular. The native - PAGE analysis showed that it possessed three kinds of laccase isoenzymes. The analysis on the enzymatic properties showed that the laccase activity arrived at the highest level of 382. 6 U/L after Dimao 1 strain was fermented for 11 days in liquid PDA medium, and the optimum temperature and pH value were 50℃ and 3.2 respectively for laccase activity. The laccase production of Dimao 1 was more stable to different temperatures and pH values.

关 键 词：黑木耳 漆酶 酶学性质 

分 类 号：S646.601[农业科学—蔬菜学]