siRNA对脂多糖诱导下HepG2细胞caspase-8基因的干扰及对凋亡的影响  

Effects of siRNA targeting casepase-8 gene on apoptosis of HepG2 cells with LPS induced

作　　者：江宇[1] 董培红[1] 陈迎晓[1] 龚玲[1] 李骥[1] 陈永平[1] 

机构地区：[1]温州医学院附属第一医院感染科,浙江温州325000

出　　处：《中西医结合肝病杂志》2010年第3期153-155,192,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：温州市科技局课题(No.Y20060294)

摘　　要：目的:研究针对人caspase-8基因mRNA的siRNA(small interfering RNA)表达载体,观察其转染人肝癌细胞HepG2后,细胞caspase-8基因表达的变化,及其对细胞凋亡的影响。方法:采用针对人caspase-8基因mRNA的siRNA表达质粒,由脂质体介导瞬时转染HepG2细胞株,并采用脂多糖(LPS)诱导HepG2细胞凋亡,RT-PCR、Westernblot测定caspase-8表达。结果:针对人caspase-8基因mRNA的siRNA表达质粒能抑制caspase-8mRNA和蛋白表达;转染后HepG2细胞体外生长明显受到抑制。结论:siRNA表达质粒能有效地抑制HepG2细胞中caspase-8的表达,抑制细胞凋亡并促进生长。为进一步探究肝衰竭基因治疗提供了实验依据。Objective：To investigate the effects of siRNA targeting casepase-8 gene and to observe its influence on apoptosis of HepG2 cells after transfected into cells.Methods：siRNA were transiently transfected into HepG2 cells via cathodolyte liposome transfectiojn method.After induced with LPS,the interference effect was detected by RT-PCR and Western blot.Results：siRNA could restrain the casepase-8 mRNA and protein express,and restrain the cells graning.Conclusion：The constructed siRNA expressing plasmid can reduce the expression of caspase-8,inhibit the proliferation of HepG2 and induce apoptosis.The experiment provides the foundation for gene therapy of hepatic failure.

关 键 词：SIRNA CASPASE-8 凋亡 

分 类 号：R575[医药卫生—消化系统]