大剂量维生素C对胰腺癌PANC-1细胞侵袭能力及MMP2表达的影响  

Effect of high-dose vitamin C on MMP2 expression and invasive ability in human pancreatic cancer cell line PANC-1

作　　者：陈雄[1] 孙维佳[1] 廖洁[1] 段小辉[1] 陆晔斌[1] 

机构地区：[1]中南大学湘雅医院普外科,,长沙410008

出　　处：《中国医师杂志》2010年第6期736-739,共4页Journal of Chinese Physician

摘　　要：目的 探讨大剂量维生素C对胰腺癌细胞株PANC-1细胞基质金属蛋白酶2（MMP2）的表达和侵袭能力的影响,为临床维生素C治疗胰腺癌可行性提供理论依据.方法 采用transwell侵袭实验比较不同浓度维生素C组PANC-1细胞的侵袭能力,并运用RT-PCR和Western b1ot检测并比较各组细胞MMP2 mRNA和蛋白水平的表达情况.结果 1.0、5.0和10.0 mM浓度维生素C处理组MMP2 mRNA的相对丰度值较空白对照组显著降低（0.510±0.004 vs 0.792±0.006,0.391±0.007 vs 0.792±0.006,0.282±0.008 vs 0.792±0.006,P＜0.05）.1.0、5.0和10.0 mM浓度维生素C处理组MMP2蛋白的相对丰度值较空白对照组显著降低（0.519±0.004 vs 0.761±0.014,0.310±0.007 vs 0.761±0.014,0.297±0.008 vs 0.761±0.014,P＜0.05）.1.0、5.0和10.0 mM浓度维生素C处理组穿过transwell的细胞数相较空白对照组显著降低（452±22 vs 653±28,340±32 vs 653±28,409±33 vs 653±28,P＜0.05）.结论 大剂量维生素C可以降低PANC-1细胞MMP2的表达,并可减弱其侵袭能力.Objective To observe the effect of high-dose vitamin C on MMP2 expression and invasive ability in PANC-1. Methods Transwell invasion assay was used to compare the invasive ability of PANC-1 cells in different concentrations of vitamin C treated groups. RT-PCR and Western blot were used to detect and compare the levels of MMP2 mRNA and protein expression in each group. Results Compared with the 0mM vitamin C treated group, the mRNA expression of MMP2 was significantly decreased in 1.0, 5.0,10.0mM group（0. 510 ±0. 004 vs 0. 792 ±0. 006, 0. 391 ±0. 007 vs 0. 792 ±0. 006, 0. 282 ±0. 008 vs 0. 792 ± 0. 006, P 〈 0.05）. Compared with the 0raM vitamin C treated group, the protein expression of MMP2 was significantly decreased in 1.0,5.0,10. 0raM group（0. 519 ± 0. 004 vs 0. 761 ± 0. 014,0. 310 ± 0. 007 vs 0. 761±0. 014,0. 297 ±0. 008 vs 0. 761 ±0. 014, P 〈0. 05）. Compared with the 0mM vitamin C treated group, the invaded cell number was significantly decreased in 1.0,5.0,10. 0mM groups（452 ± 22 vs 653 ± 28,340 ± 32 vs 653 ± 28,409 ± 33 vs 653 ± 28, P 〈 0. 05 ）. Conclusion High-dose vitamin C can decrease the expression of MMP2 in PANC-1 cells, and weaken its invasive ability.

关 键 词：抗坏血酸/投药和剂量 胰腺肿瘤/病理学/代谢 肿瘤侵润 基质金属蛋白酶2/代谢/药物作用 

分 类 号：R735.9[医药卫生—肿瘤]