Kras基因与辐射诱发小鼠胸腺淋巴瘤的相关性研究  

Study on relevance between kras gene and thymic lymphomas of mice induced by X-rays

作　　者：于雷[1] 孙世龙[1] 方芳[1] 刘永哲[1] 巩宏伟[1] 石磊[1] 鞠桂芝[1] 

机构地区：[1]吉林大学公共卫生学院,长春130021

出　　处：《辐射研究与辐射工艺学报》2010年第3期190-192,共3页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金项目(30670630);吉林大学研究生创新基金项目(20091032)资助

摘　　要：为探讨kras基因与辐射诱发小鼠胸腺淋巴瘤的关系,先采用X射线照射BALB/c小鼠建立胸腺淋巴瘤模型,取胸腺淋巴瘤和正常胸腺,分别提取总RNA、合成cDNA和提取总蛋白,采用实时定量PCR(Quantitative realtime PCR,QRT-PCR)和Western blot法对kras基因mRNA和蛋白表达分别进行检测。结果表明,胸腺淋巴瘤kras基因mRNA表达水平(2.66±1.51)比正常胸腺组织(0.93±0.30)明显增高,两组比较有统计学意义(p<0.05),胸腺淋巴瘤kras基因蛋白表达水平也高于正常胸腺组织。结果提示,kras基因与辐射诱发小鼠胸腺淋巴瘤密切相关,可能是辐射致癌的一种易感基因。In order to investigate the relation between kras gene and thymic lymphomas of mice induced by X-rays, we made the thymic lymphomas model of BALB/c mice exposed to X-rays, so as to extracte total RNA and synthesize cDNA and total protein from both of thymic lymphomas and normal thymus tissue. The mRNA and pro- tein expression of kras gene in thymic lymphomas and normal thymus were detected by QRT-PCR and Western blot, respectively. The results show that mRNA expression of kras gene in thyrnic lymphomas （2.66 ~ 1.51） is significantly higher （p〈0.05） than that of normal thymus tissue （0.93：~0.30）; and protein expression of kras gene in thymic lym- phomas is higher than that of normal thymus tissue. The results suggest that kras gene might be relative to thymic lymphomas induced by X-rays, and it might be a susceptibility gene to radiation carcinogenesis.

关 键 词：胸腺淋巴瘤 KRAS基因 电离辐射 实时定量PCR 

分 类 号：Q345[生物学—遗传学] R733[医药卫生—肿瘤]