小分子糖负载血小板+冻干保护剂混合悬液的相变点测定与屏蔽实验研究  

The phase transformation point of small molecule carbohydrate loaded platelet and anti-freeze drying reagent suspension

作　　者：王捷熙[1] 杨超[1] 王艳[1] 刘敏霞 刘茜[2] 房磊[1] 杜为[1] 高峰[1] 权国波[1] 韩颖[1] 

机构地区：[1]军事医学科学院野战输血研究所,北京100850 [2]北京市红十字血液中心

出　　处：《中国输血杂志》2010年第5期338-340,共3页Chinese Journal of Blood Transfusion

基　　金：军队"十一五"重大专项课题资助(06Z076)

摘　　要：目的测定冻干血小板长期保存预冻程序和所依据的制品相变点温度并摸索相应屏蔽相变点的最佳冷冻曲线。方法研制出小分子糖负载血小板+冻干保护剂混合悬液,使用速率程序降温仪分析测定该混合悬液样品的相变点温度;根据测得的相变点温度,设计屏蔽相变点的预冻程序,根据温度变化曲线,判断相变点屏蔽结果。结论该混合悬液样品的相变点温度为(-13.56±2.05)℃;据此相变点温度所设计的3种相应的屏蔽相变点的预冻程序中,程序3为最佳冷冻曲线。结果获得了小分子糖负载血小板+冻干保护剂混合悬液的相变点温度及相应的屏蔽相变点的最佳预冻程序。Objective To study a freeze drying technique for longer period platelet preservation.Methods There are three important processes of platelet lyophilized preservation, i.e. the pretreatment, the pre-freeze and vacuum desiccation. The phase transformation point was the key for protection of pre-freeze procedure. In this study, the transformation point of small molecule carbohydrate loaded platelets and anti-freeze drying reagent suspension were determined by descending temperature regularity method. Results The transformation point of sample was （-13.56±2.05）℃.We designed three pre-freeze procedures according to transformation point, and selected procedure 3 as the pre-freeze procedure of platelet lyophilization preservation studies. Conclusion The phase transformation point, and the optimal procedure were acquired.

关 键 词：小分子糖负载血小板 冻干保护剂 混合悬液 相变点温度 屏蔽相变点 

分 类 号：R457.12[医药卫生—治疗学] O552.6[医药卫生—临床医学]