昆布多糖硫酸酯对PC-3细胞PTEN和P27kip1基因表达的影响  

Effects of laminarin sulphate on the expressions of PTEN and P27kip1 in prostate cancer PC-3 cells

作　　者：邹明畅[1] 崔飞伦[1] 盛玉青[1] 邱镇[1] 

机构地区：[1]江苏大学附属人民医院药剂科,江苏镇江212002

出　　处：《中华男科学杂志》2010年第6期498-503,共6页National Journal of Andrology

基　　金：镇江市科学技术局资助项目(SH2007027)~~

摘　　要：目的:研究昆布多糖硫酸酯(LAMS)对体外培养的非激素依赖型人前列腺癌细胞株PC-3的作用,以及对PC-3细胞PTEN和P27kip1基因表达的影响,并探讨其抗肿瘤的作用机制。方法:分别用0、50、100、200μg/ml浓度的LAMS作用于PC-3细胞,用WST-8法检测其对细胞生长的抑制作用,利用流式细胞术(FCM)分析细胞周期和凋亡的变化,用荧光显微镜观察PC-3细胞形态的变化,RT-PCR和Western印迹检测细胞增殖、凋亡相关基因PTEN和P27kip1mRNA及蛋白的表达。结果:LAMS能抑制PC-3细胞的增殖,呈时间-剂量效应;不同浓度LAMS诱导PC-3细胞出现剂量依赖性S期阻滞;LAMS作用于PC-3细胞后凋亡率增加,并出现凋亡的形态学改变,相关基因PTEN和P27kip1mRNA及蛋白的表达呈剂量依赖性增加。结论:LAMS能抑制体外PC-3细胞的生长,并促进其S期阻滞和凋亡,PTEN和P27kip1mRNA及蛋白的表达明显增加,可能是其抑制前列腺癌生长的机制之一。Objective： To study the effect of laminarin sulphate （LAMS） on the expressions of PTEN and P27kip1 in androgenindependent prostate cancer PC-3 cells in vitro, and investigate the mechanism of its anti-tumor action. Methods： The inhibitory effects of different concentrations of LAMS （0, 50, 100, 200 μg/ml） on androgen-independent prostate cancer PC-3 cells were detected by WST-8 assay. The morphology of PC-3 cells was observed under the fluorescence microscope, and the cell cycle and apoptosis were analyzed by flow cytometry. The mRNA and protein levels of PTEN and P27kip1 were measured by RT-PCR and Western blot. Results： LAMS inhibited the proliferation of androgen-independent prostate cancer PC-3 cells in a dose- and time-dependent manner, and the cell cycle analysis showed that PC-3 cells were arrested in the S phase after treated with different concentrations of LAMS. The rate of apoptosis was increased and many typical apoptotie morphological features were observed under the fluorescence microscope. The PTEN and P27kip1 expressions at mRNA and protein levels were increased in a dose-dependent manner. Conclusion ： LAMS can inhibit the proliferation, arrest the cell cycle in the S phase and induce apoptosis of prostate cancer PC-3 ceils. The significantly increased expressions of PTEN and P27kip1 may be one of the mechanisms for LAMS inhibiting prostate cancer PC-3 cells.

关 键 词：前列腺癌 昆布多糖硫酸酯 PC-3细胞 PTEN P27KIP1 细胞凋亡 

分 类 号：R737.25[医药卫生—肿瘤]