联合检测诱导痰和纤支镜活组织端粒酶活性对肺癌诊断的意义  

Significance of determination of telomerase activity in induced sputum and fiberobronchoscopic biopsy samples for lung cancer diagnosis

作　　者：李红梅[1] 冯锐[2] 王子轩[3] 辛鲁群[1] 

机构地区：[1]青岛大学医学院附属医院肿瘤中心,山东青岛266003 [2]滨州医学院附属医院血液科,山东滨州256600 [3]青岛市市立医院介入科,山东青岛266011

出　　处：《山东大学学报（医学版）》2010年第6期115-117,121,共4页Journal of Shandong University:Health Sciences

基　　金：山东省医药卫生科研资助项目(2007BW09)

摘　　要：目的探讨联合检测诱导痰和纤维支气管镜活组织端粒酶活性对肺癌的诊断价值。方法采用端粒重复序列扩增-酶联免疫吸附实验方法 ,对80例肺癌患者和50例肺部良性病变患者,分别检测其诱导痰和纤维支气管镜活组织端粒酶的活性。结果肺癌患者诱导痰和纤维支气管镜活组织端粒酶的活性均明显高于肺部良性病变患者(P<0.001),不同病理类型的肺癌患者两种标本的端粒酶活性差异无统计学意义(P>0.05)。诱导痰和纤维支气管镜活组织端粒酶活性的检测对肺癌的诊断敏感性、特异性、准确性分别为62.5%(50/80)和60.0%(48/80)、72.0%(36/50)和70.0%(35/50)、66.2%(86/130)和63.8%(83/130);两项联合检测的敏感性、特异性和准确性分别为82.5%(66/80)、64.0%(32/50)和75.4%(98/130)。结论诱导痰和纤维支气管镜活组织端粒酶活性的联合检测具有更高的敏感性,可以提高肺癌的诊断率。Objective To investigate the significance of determination of telomerase activity in induced sputum and fiberobronchoscopic biopsy samples in lung cancer patients. Methods The technique of TRAP（telomeric repeat amplification protocal）-PCR-ELISA was employed to detect telomerase levels in induced sputum and fiberobronchoscopic biopsy samples in 80 lung cancer patients and 50 benign lung disease patients. Results Telomerase levels in induced sputum and fiberobronchoscopic biopsy samples were higher in patients with lung cancer than in those with benign lung disease（P0.001）.There was no significant difference in telomerase activity between the different pathological types（P0.05）.The sensitivity,specificity and overall accuracy of telomerase activity was 62.5% （50/80）,72.0 % （36/50） and 66.2%（86/130） in induced sputum,and 60.0%（48/80）,70.0%（35/50）,and 63.8%（83/130） in fiberobronchoscopic biopsy samples respectively. The sensitivity,specificity and overall accuracy of telomerase activity simultaneously determined by the above methods was 82.5%（66/80）,64.0%（32/50）and 75.4%（98/130） respectively. Conclusion Determination of telomerase activity in induced sputum and fiberobronchoscopic biopsy samples has high sensitivity and can improve the diagnostic accuracy for lung cancer.

关 键 词：端粒 末端转移酶 痰 支气管镜 肺肿瘤 诊断 

分 类 号：R734.2[医药卫生—肿瘤]