多核白细胞弹性蛋白酶对人角膜基质细胞MMP-2、MMP-9表达的影响  

Effects of neutrophil elastase on expression of the MMP-2,9 in cultured human corneal fibroblasts

作　　者：刘泊明[1] 张冰洁[2] 杨贲[2] 郝继龙[2] 

机构地区：[1]伊通满族自治县第一人民医院眼科,吉林伊通130700 [2]吉林大学中日联谊医院眼科,吉林长春130033

出　　处：《中国实验诊断学》2010年第6期855-857,共3页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金资助项目(30772384)

摘　　要：目的观察多核白细胞弹性蛋白酶与白细胞介素-1α对人角膜基质细胞表达的MMP-2、MMP-9的影响,进而探讨角膜溃疡的发生机理。方法体外培养人角膜基质细胞,在培养液中添加或不添加多核白细胞弹性蛋白酶或白细胞介素-1α,培养5天,通过明胶酶谱分析的方法测定培养上清中的MMP-2和MMP-9的表达。结果体外培养的人角膜基质细胞无任何刺激下可释放MMP-2;白细胞介素-1α可加强并激活MMP-2的表达,同时可诱导MMP-9前体的释放;多核白细胞弹性蛋白酶在白细胞介素-1α诱导下可完全激活前体MMP-9转化为活化形式。结论多核白细胞弹性蛋白酶和白细胞介素-1α协同作用参与角膜溃疡的发生。Objective To understand the mechanism of corneal ulceration, we investigated the role of neutrophil elastase （NE）, interleukin-1α（IL-1α） and corneal fibroblasts on expression of the matrix metalloproteinases（ MMP）-2,9 in cultured human corneal fibroblasts. Methods NE or IL-1α was added to the culture medium of human corneal fibroblasts 5 day later. The activity of MMP-2 and MMP-9 were examined by Gelatin zymography. Results Cultured human corneal fibroblasts released MMP-2 without any stimulation. IL-1α enhanced and activated the expression of MMP-2, while induced the release of proMMP-9. NE transformed proMMP-9into active fomts induced by IL-1α. Conclusion NE and IL-lacan promote human corneal fibroblasts in collagen degradation,the two are synergistic.

关 键 词：多核白细胞弹性蛋白酶 白细胞介素-1Α 角膜基质细胞 MMP-2 MMP-9 

分 类 号：R772.21[医药卫生—眼科]