志贺氏菌芳香族氨基酸缺陷减毒株的构建  

Construction of an aromatic amino acid attenuated strain

作　　者：刘英杰[1] 芮贤良[1] 徐永强[1] 苏国富[1] 

机构地区：[1]军事医学科学院生物工程研究所,天津300162

出　　处：《武警医学》1999年第2期68-71,共4页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的构建一种新型痢疾疫苗，使其较完整地保留保护性抗原，又不致丧失其侵袭力。方法用PCR技术从野生型福氏2a志贺氏菌2457T中克隆出aroA基因，在体外进行精确的缺失突变，并通过体内同源重组，构建成ΔaroA突变体RS426。结果这种突变体仍保持了侵袭能力和保护性O抗原的表达，但其毒力已明显降低，不能产生豚鼠角结膜炎，小鼠半数致死量明显提高。结论免疫保护试验显示，RS426可在小鼠中产生对福氏2a野生菌100％的保护作用。Objective Shigella spp 'an ic appropriately attenuated by the ~ gene mutation. Method The ~ mutant may beused as a new generation canclidate anti -- shigella vaccine. The areA gene of S. flexneri 2 a 2457 T was alnphoed by aiR and mutatedby Pnyise deletion of a driddle 4'f fragment of the ~A gene in vial. The constrUcted boA gene was ugh to replace the wild -- acamA gene of S. fie^ri 2 a 2457T I)\ honlologous recolnbination which resulted in the constrUction of a algA mutant RS 426. RedtSExperimental results showed that illis strain retained the ability of cell invasion and 0 antigen expression, but ho adn st^canily attenuated, Conclndon That is codrirmed h}' iEs loss of the ability' to provoke the keratoconjunctivitis in tinea -- pig and much higherLDro value in face.

关 键 词：志贺氏痢疾杆菌 aroA基因 同源重组 细菌性痢疾 

分 类 号：R516.402[医药卫生—内科学]