气相色谱法测定人参提取物中15种有机氯农药残留量  

Detection of residues of fifteen pesticides in Ginseng extract by gas chromatography

作　　者：严付华[1] 张艳[1] 曾建国 

机构地区：[1]长沙市疾病预防控制中心,湖南长沙410001 [2]湖南美可达生物资源有限公司,湖南长沙410016

出　　处：《中国热带医学》2010年第8期1016-1017,1023,共3页China Tropical Medicine

摘　　要：目的研究毛细管气相色谱法测定人参提取物中15种有机氯农药残留的方法。方法样品经石油醚为提取溶剂振荡提取后,经Florisil小柱净化,以毛细管气相色谱测定。结果实验结果表明在线性范围内相关系数r2>0.992,平均加标回收率为71.3%～103.9%,相对标准偏差为(RSD)为2.1%～11.0%,检出限为0.1～1.1μg·kg-1。结论研究方法能满足农药残留测定的要求。Aim To detect multiresidue method based on solid-phase extraction was developed for the simultaneous detection of 15 pesticides （pentachlorobenzene,tecnazene,tetrachloroanilline,HCB,alpha-BHC,gamma-BHC,Beta-BHC, pentachloroanilline,Delta-BHC,methyl-pentachlorophenyl sulfide （MPCPS）,procymidone,p,p＇-DDE,p,p＇-DDD,o,p＇-DDT, p,p＇-DDT） in Ginseng extracts. Methods The extraction procedure was carried out on Florisil columns preconditioned with petroleum ether. The subsequent elution of pesticides was performed with a mixture of petroleum ether-ethyl acetate（9：1, v/v） prior to the determination by gas chromatography with electron capture detection. Results Good resolution of all compounds was achieved. Detection limits were between 0.1 μg/kg to 1.1 μg/kg,respectively,with linear calibration curves up to 0.2 mg·L^-1 for most constituents. Average recovery for all the pesticides studied were between 71.3% and 103.9%. Conclusion The proposed method is suitable for for detection of pesticide residues in Ginseng extracts.

关 键 词：毛细管气相色谱法 固相萃取 有机氯 人参提取物 

分 类 号：R155.51[医药卫生—营养与食品卫生学]