天然气库上方土壤微生物群落研究  

Study on Microbial Community in Soil Above A Gas Reservoir

作　　者：张凡[1] 佘跃惠[2] 孔淑琼[2] 舒福昌[2] 喻高明[2] 侯读杰[1] 

机构地区：[1]中国地质大学(北京)能源学院,北京100080 [2]长江大学化学与环境工程学院,湖北荆州434023

出　　处：《化学与生物工程》2010年第6期54-58,共5页Chemistry & Bioengineering

基　　金：中石油集团公司中青年创新基金资助项目(07E1025)

摘　　要：通过基于16S rRNA基因克隆文库的方法研究了天然气库上方土壤的微生物群落结构。针对大港油田板876气库上方同一取样点1.0 m和2.0 m的土壤样品构建了16S rDNA克隆文库DGS1和DGS2,并对其150个阳性克隆进行限制性酶切片段长度多样性分析(ARDRA)。结果表明,克隆文库DGS1有40个操作分类单元(OTU),克隆文库DGS2有39个OTU,该天然气库上方土壤中微生物较丰富;但由于土壤深度的不同两个土壤样品微生物群落结构存在着差异。每个OTU的代表克隆序列分析结果表明,克隆文库DGS1中优势菌群为芽单胞菌(Gemmatimonadetes)28%、绿弯菌(Chloroflexi)23%和放线菌(Actinobacterium)21%;克隆文库DGS2菌群分布较平均,其中Chloroflexi19%、硫氧化菌(Sulfur-oxidizing)12%、酸酐菌(Acidobacteria)10%、Gemmatimonadetes10%。天然气库上方土壤微生物多样性的分子分析可为开展微生物油气勘探(MPOG)技术奠定基础。16S rRNA gene clone library construction was used to analyze the microbial communities of soil sampled from above a known Ban876 gas reservoir.Two clone libraries DGS1 and DGS2 were constructed.There were 150 clones from the two libraries respectively for amplified ribosomal DNA restriction analysis（ARDRA）.40 Taxanomic operational units（OTUs） were found in library DGS1,and 39 OTUs were found in library DGS2.Sequence analysis of the representative clone of each OTU showed that dominant bacteria of the DGS1 were affiliated with Gemmatimonadetes 28%,Chloroflexi 23%,Actinobacterium 21%;and Chloroflexi 19%,Sulfur-oxidizing 12%,Acidobacteria 10%,Gemmatimonadetes 10% for DGS2,which provides foundation for application of MPOG（Microbial prospecting of oil and gas）.

关 键 词：气库 16SRRNA基因 克隆文库 微生物群落 微生物油气勘探(MPOG) 

分 类 号：Q939[生物学—微生物学] Q785