环境激素双酚A与DNA相互作用的电化学研究  

Study on Interaction of Environmental Hormone Bisphenol A With DNA by Electrochemical Method

作　　者：王辰[1] 付艳丽[1] 刘晓海[2] 高云涛[1] 

机构地区：[1]云南民族大学化学与生物技术学院,云南昆明650031 [2]云南省环境科学研究院,云南昆明650034

出　　处：《环境科学导刊》2010年第4期5-8,共4页Environmental Science Survey

基　　金：云南省中青年学术技术带头人后备人才计划项目(2006py01-41)

摘　　要：采用电化学方法研究了双酚A与DNA间的相互作用。结果表明,DNA的存在使双酚A的氧化峰电流减小,且峰电位正移,双酚A与DNA的相互作用以嵌插作用为主。双链DNA(dsDNA)与双酚A的结合大于单链DNA(ssDNA),结合比为1∶4,结合常数β为1.35×1023。The interaction between bisphinol A and DNA was investigated by electrochemical method.The results indicated that the oxidation peak currents of bisphenol A decreased and the peak potentials also shifted positively when adding DNA.Bisphenol A reacts with DNA mainly in a mode of intercalation.The interaction between bisphenol A and dsDNA was stronger than that of ssDNA.The combining ratio of bisphenol A to dsDNA was 1：4 with the combining constant as 1.35×10^23.

关 键 词：双酚A DNA 循环伏安法 相互作用 

分 类 号：X17[环境科学与工程—环境科学]