金属基底合金浸提液影响人牙龈成纤维细胞MMP-1、MMP-3表达的实验研究  

The Effects of Base Metal Alloy Crown Extract on Human Gingival Fibroblast MMP-1,MMP-3 Expression Levels

作　　者：刘淼[1] 黄克强[1] 赵芳英[1] 贺成功[1] 

机构地区：[1]辽宁医学院,辽宁锦州121000

出　　处：《辽宁医学院学报》2010年第3期203-205,I0002,共4页Journal of Liaoning Medical University (LNMU) Bimonthly

摘　　要：目的观察不同种类烤瓷冠金属基底合金浸提液对人牙龈成纤维细胞中MMP-1、MMP-3表达水平的影响。方法制备镍铬合金、纯钛、金铂合金金属浸提液,组织块法培养人牙龈成纤维细胞,等量金属浸提液置换细胞培养液24 h,分别在1、6、13、24 h收集上清液,ELISA法测定MMP-1、MMP-3表达水平。结果(1)MMP-1、MMP-3在镍铬合金组的表达水平明显高于其他实验组及对照组,差异有统计学意义(P<0.01)。(2)MMP-1、MMP-3在纯钛、金铂合金组的表达水平与空白组有差异,但差异无统计学意义(P>0.05)。结论镍铬合金对人牙龈成纤维细胞损伤大,纯钛、金铂合金具有良好的生物相容性。Objective To observe the effects of different types of base metal alloy crown extract on human gingival fibroblast MMP-1,MMP-3 expression levels.Methods Preparation of nickel-chromium alloy,titanium,gold platinum alloy metal extracts.In vitro culture of human gingival fibroblasts,extract equivalent metal replacement cell culture medium 24 h,at 1 h,6 h,13 h,24 h supernatant.Results The expression of MMP-1,MMP-3 in nickel-chromium alloy group was significantly higher than other experimental group and control group,statistical significance（P〈0.01）.The expression of MMP-1,MMP-3 inpure titanium and gold platinum alloy group is different from the control group,but no statistical significance（P〉0.05）.Conclusions Nickel-chromium alloy do human gingival fibroblasts a serous injury.Pure titanium and gold platinum alloys have good biocompatibility.

关 键 词：人牙龈成纤维细胞 烤瓷熔附金属全冠 基底金属浸提液 基质金属蛋白酶 

分 类 号：R783.4[医药卫生—口腔医学]