重氮乙酰丝氨酸诱导大鼠胰腺腺泡细胞癌癌前病变及其K-ras基因突变的实验研究  

THE EXPERIMENTAL STUDY OF PRECANCEROUS LESION OF PANCREATIC ACINAR CELLULAR CANCER IN RATS INDUCED BY AZASERINE AND THE DETECTION OF K RAS MUTATION

作　　者：徐峰[1] 曹伟[2] 寿楠海[1] 姜希宏[1] 徐克森[1] 

机构地区：[1]山东医科大学附属医院普外科 [2]山东医科大学基础医学院药理学教研室,济南250012

出　　处：《癌变．畸变．突变》1999年第2期80-85,共6页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：建立大鼠胰腺腺泡细胞癌癌前病变的动物模型，了解该癌前病变及Ｋｒａｓ 基因突变的情况　雄性Ｗｉｓｔａｒ 大鼠单次腹腔注射重氮乙酰丝氨酸３０ｍｇ／Ｋｇ 体重，４ 个月后常规取胰腺组织行光镜和电镜检查，并应用ＰＣＲＳＳＣＰ法检测Ｋｒａｓ 基因突变情况　大鼠胰腺腺泡细胞癌癌前病变发生率１００％ ，Ｋｒａｓ 基因突变率４２－１ ％ 。重氮乙酰丝氨酸可成功诱导大鼠胰腺腺泡细胞癌癌前病变，Ｋｒａｓ 基因突变在此过程中属早期事件。To set up the animal model of precancerous lesion of rat's pancreatic acinar cellular cancer, learn its biological feature and K ras gene code 12 mutation. Male Wistar rats were injected Azaserine 30mg/kg(ip). 4 months later, rats' pancreas were autopsied and K ras gene code 12 mutation were detected by PCR SSCP. The incidence of precancreous lesion of rats' pancreatic acinar cellular cancer was 42.1%.Azaserine could induce precancerous lesion of rats' pancreatic acinar cancer successfully, K ras gene code 12 mutation was early change during this process.

关 键 词：重氮乙酰丝氨酸 胰腺肿瘤 K-RAS基因 癌前病变 

分 类 号：R735.902[医药卫生—肿瘤]