GRIM-19及其靶基因产物STAT3与子宫颈癌的相关性  

Correlation between GRIM-19 and its target gene product STAT3 and cervical carcinoma

作　　者：卫莹[1] 凌斌[1] 李敏[1] 朱靖[1] 范少君[1] 冯定庆[1] 肖卫华[2] 周颖[1] 

机构地区：[1]安徽医科大学附属省立医院妇产科分子实验室.安徽省分子医学重点实验室 [2]中国科技大学生命科学院免疫学研究所

出　　处：《中国妇幼保健》2010年第18期2559-2561,共3页Maternal and Child Health Care of China

基　　金：安徽省自然科学基金项目〔090413117〕

摘　　要：目的:通过研究干扰素/维甲酸联合应用诱导细胞凋亡相关基因19(GR IM-19)及其靶基因产物转录信号子与激活子3(STAT3)在子宫颈癌组织中的表达情况,探讨GR IM-19及其靶基因产物在子宫颈癌发病中的作用。方法:采用W estern B lotting和RT-PCR方法检测40例子宫颈癌组织和30例正常子宫颈组织中GR IM-19与STAT3及下游基因的表达情况。结果:在子宫颈癌组织中STAT3 mRNA与蛋白均高表达,STAT3下游基因Cyc linB1和Bc l-2L1 mRNA高表达,而GR IM-19mRNA及蛋白表达在癌组织中均明显低于正常组织(P<0.05)。结论:GR IM-19与STAT3在子宫颈癌组织中表达呈负相关,GR IM-19在子宫颈癌组织中的低表达可能与子宫颈癌的发生与发展密切相关。Objective：To explore the function of GRIM-19 and its target gene product STAT3 in pathogenesis of cervical carcinoma by investigating expressions of GRIM-19 and its target gene product STAT3 in cervical carcinoma induced by interferon combined with retinoic acid.Methods：The expression levels of GRIM-19,STAT3 and its downstream genes in 40 cases with cervical carcinoma and 30 cases with normal cervical tissues were detected by Western Blotting and RT-PCR.Results：The expression levels of STAT3 mRNA and protein were high in cervical carcinoma tissues,the expression levels of STAT3 downstream genes（CyclinB1 and Bcl-2L1） mRNA were high,the expression levels of GRIM-19 mRNA and protein in cervical carcinoma tissues were significantly lower than those in normal cervical tissues（P〈0.05）.Conclusion：There is a negative correlation between GRIM-19 expression and STAT3 expression in cervical carcinoma,low expression of GRIM-19 in cervical carcinoma may be related to occurrence and development of cervical carcinoma.

关 键 词：子宫颈肿瘤 GRIM-19基因 STAT3基因 

分 类 号：R737.33[医药卫生—肿瘤] R73-36[医药卫生—临床医学]