大蒜精油抑制白细胞介素-1α诱导的单核细胞-内皮细胞粘附及机理探讨  

Study on Effect and Mechanism of Essential Garlic Oil in Inhibiting Monocyte Vascular Endothelial Cell Adhesion Induced by Interleukin 1α

作　　者：葛璐璐[1] 张薇[1] 戴云[1] 臧燕 黄纯洁 

机构地区：[1]第一军医大学组织胚胎学教研室

出　　处：《中国中西医结合杂志》1999年第3期152-154,共3页Chinese Journal of Integrated Traditional and Western Medicine

摘　　要：目的：观察大蒜精油（ＥＧＯ）对白细胞介素－１α（ＩＬ－１α）诱导的人脐静脉内皮细胞（ＨＵＶＥＣ）上血管细胞粘附分子－１（ＶＣＡＭ－１）表达及单核细胞粘附率的影响。方法：胰酶消化法分离ＨＵＶＥＣ，用Ｕ９３７单核细胞与之联合培养，分别加入ＩＬ－１α和ＥＧＯ加ＩＬ－１α孵育，计算单核细胞－内皮细胞粘附率，ＶＣＡＭ－１免疫荧光染色，粘附细胞仪（ＡＣＡＳ５７０）测定平均荧光值。结果：单独培养及联合培养的情况下，ＥＧＯ均对ＩＬ－１α诱导的ＨＵＶＥＣ上ＶＣＡＭ－１表达具有明显的抑制作用，并能显著下调ＩＬ－１α作用下单核细胞内皮细胞的粘附。结论：ＥＧＯ具有抗单核细胞与血管内皮细胞粘附的作用，其机理可能是通过抑制内皮细胞表面粘附分子的表达。Objective: To observe the effects of essential garlic oil (EGO) on vascular cell adhesive molecule 1 (VCAM 1) expression of endothelial cell and monocyte adhesion induced by interleukin-1α (IL-1α). Methods: Human umbilical vein endothelial cells (HUVEC) were isolated by pancreatin digestion method and co cultured with U937 monocyte and IL-1α or EGO+IL-1α respectively. The adhesion rate of monocyte to endothelial cell was measured and mean fluorescent intensity of immuno stained of VCAM 1 was determined by ACAS 570. Results: EGO showed significant inhibition on VCAM 1 expression induced by IL-1α either in simple culture with HUVEC or in co culture with HUVEC+U937, and could obviously down regulate the monocyte endothelial cell adhesion under effect of IL-1α. Conclusion: EGO has the effect of antagonizing against adhesion of monocyte and vascular endothelial cell, it may be due to the inhibition of EGO on adhesive molecular expression on endothelial cell.

关 键 词：大蒜精油 内皮细胞 VCAM-1 IL-1Α 单核细胞 

分 类 号：R282.710.5[医药卫生—中药学] R285.5[医药卫生—中医学]