中国大陆庚型肝炎病毒NS区部分基因序列分析及变异的初步研究  

Preliminary study on NS5 region gene sequencing and variants of hepatitis G virus in China

作　　者：李伯安[1,2] 董志锋[1,2] 程云[1,2] 高蓉[1,2] 施红[1,2] 郭仁峰 薛净[1,2] 罗清华 韩凤连[1,2] 张建宗 

机构地区：[1]解放军302医院免疫研究室 [2]中国生物工程开发中心

出　　处：《中华实验和临床病毒学杂志》1999年第1期74-76,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的研究中国庚型肝炎患者分离的病毒基因的特异性及变异规律。方法应用逆转录－巢式聚合酶链反应（ＰＣＲ）技术从１６例庚型肝炎病毒（ＨＧＶ）感染住院病人血清中扩增了ＨＧＶＮＳ５区部分基因片段（１４１ｎｔ）。经纯化后采用双脱氧链末端终止法进行序列分析。结果观察到在ＨＧＶＮＳ５区第７８２０－７９６０核苷酸之间的５′端较３′端核苷酸及其编码氨基酸变异率高。与Ｌｉｎｎｅｎ等报道的美国株ＨＧＶ相比，中国人ＨＧＶＮＳ５区核苷酸的变异主要集中在ＨＧＶＮＳ５工第７８２４，７８４５～７８６０，７８９２，７９１４，７９１７和７９３５核苷酸位点及区域，各个核苷酸位点的变异率在０～８９．５％，与美国株相比核苷酸序列的同源性为８３．６％～９５．７％，平均８９．６％；氨基酸为８３．０％～１００％，平均为９１．２％。在７９３３核苷酸位点与美国株相比变异率为９３．７％。结论中国人群之间ＨＧＶＮＳ５区部分基因及其推测编码蛋白较保守，但与美国株有明显差别。不同硷基在序列中呈散在分布，而在某些位点虽与美国株相比变异很大，但中国人群之间离散度较小，ＨＧＶ核酸的变异可能与地域因素有关。Objective To study the genomic specificity and variation of Hepatitis G virus(HGV) in China. Methods The 141nt cDNA fragments locating at nonstructural region 5 (NS5) of HGV were obtained from the sera of 16 HGV infected patients by RT nested PCR using NS5 primers, then purified and directly sequenced by dideoxynucleotide chain termination method.Results The sequence diversity near the N terminus of the region between nt 7 820 and 7 960 was more marked than that near the C terminus. Compared with the HGV US isolate ,the nt alterations of NS5 in Chinese isolates usually took place at nt 7 824, 7 892, 7 914, 7 917, 7 935 and in region nt7 845 7 860, the rate of variability at one nt position was 0 89.5%. The nucleotide homology was 83.6% 95.7%(89.6% in average) and the homology of deduced amino acid sequence was 83.0 100%(91.2% in average).Conclusion The nucleotide and deduced amino acid sequences of NS5 of Chinese HGV are relatively conserved but are obviously different from the HGV and GBV C reported in USA in this study.

关 键 词：庚型肝炎病毒 序列同源性 核酸 氨基酸 PCR 

分 类 号：R373.21[医药卫生—病原生物学]