乳腺癌组织PIGF表达及其临床意义的探讨  

Expression and significance of PIGF in human breast carcinoma tissues

作　　者：余海浪[1] 马文丽[1] 周珏宇[1] 郑文岭[2] 

机构地区：[1]南方医科大学基因工程研究所,广东广州510515 [2]华南基因组中心,广东广州510800

出　　处：《中华肿瘤防治杂志》2010年第8期598-601,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：广东省重点实验室建设经费资助(2004B60144)

摘　　要：目的:研究胎盘生长因子(PIGF)在乳腺癌组织中的表达及其临床意义。方法:分别采用实时荧光定量PCR(real-time quantitative,Q-PCR)和免疫组织化学方法检测56例乳腺癌组织和72例癌旁组织中PIGF的表达。结果:Q-PCR检测乳腺癌组织中PIGFmRNA的表达水平为0.987±0.171,相应癌旁组织中PIGFmRNA的表达量为0.265±0.114;免疫组化检测乳腺癌组织中PIGF蛋白阳性率为80.4%(45/56),相应癌组织中PIGF蛋白阳性率为12.5%(8/72);癌组织中PIGF的表达均显著高于癌旁组织(P<0.05)。PIGF的高表达与淋巴结转移及Nottingham预后指数有相关性(P<0.05),但与乳腺癌的病理类型、临床分期及ER状态无相关性。结论:PIGF在乳腺癌组织中高表达,且与乳腺癌的发生、发展及临床预后密切相关。PIGF很可能在乳腺癌的发病机制中起着重要的作用。OBJECTIVE：To investigate the expression and clinical significance of placenta growth factor （PlGF） in human breast cancer.METHODS：The PlGF mRNA expression was examined quantitatively by using real-time quantitative polymerase chain reaction on a cohort of human breast cancer tissues （n=56） and corresponding adjacent tissues （n=72）,and the immunohistochemical method was used to estimate the expression of PIGF protein.RESULTS：By using Q-PCR,the expression levels of PIGF mRNA in breast cancer and adjacent tissues were 0.987±0.171 and 0.265±0.114,respectively;by using immunohistochemistry,the expression rates of PIGF protein in breast cancer and adjacent tissues were 80.4%（45/56） and 12.5%（8/72）,respectively.PlGF expression was dramatically increased in breast cancer tissues compared with the normal background breast tissue （P〈0.05）.The overexpression of PIGF in breast cancer was correlated with tumour-node-metastasis and Nottingham prognostic index （P〈0.05）,but not with other clinicopathological factors,including pathological type,clinical stage and ER.CONCLUSION：PlGF is over-expressed in breast cancer tissues and significantly correlates with clinical prognosis,and may have a pivotal role in breast cancer development and disease progression.

关 键 词：胎盘生长因子 乳腺肿瘤/病理学 实时定量PCR 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]