Neuropilin-1阳性T细胞在肿瘤免疫中的调节作用  

Regulatory effect of neuropilin-1 positive T cells in the tumor immunity

作　　者：裴向克[1,2] 蔡明[2] 周鸿敏[3] 刘畅[2] 徐利军[3] 黄建敏[3] 陈忠华 石炳毅[2] 

机构地区：[1]解放军第281医院泌尿外科,河北秦皇岛066100 [2]解放军第309医院全军器官移植中心,北京100091 [3]华中科技大学同济医学院附属同济医院器官移植研究所,湖北武汉430030

出　　处：《中华肿瘤防治杂志》2010年第9期641-643,共3页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(C0302050104);全军医学"十一五"计划科技攻关课题(06G115)

摘　　要：目的:探讨Neuropilin-1阳性T细胞(Nrp-1+T细胞)在肿瘤免疫中的调节作用。方法:通过建立B16-F10-luc-G5黑色素瘤细胞体内模型及利用活体萤光成像系统,将由小鼠脾脏细胞中分选出的Nrp-1+T细胞注射入模型小鼠体内,观察Nrp-1+T细胞对黑色素瘤细胞体内模型增殖的影响。结果:Nrp-1+T细胞占脾脏淋巴细胞的比例为(2.65±0.28)%,肿瘤模型建立6d后,Nrp-1+T细胞干预组肿瘤检测光子数高于非Nrp-1+T细胞干预组〔(3.69±0.04)×106s-1vs(1.22±0.09)×105s-1〕,两者差异有统计学意义,t=-43.438,P<0.01。结论:Nrp-1+T细胞在体内能够负性调节针对黑色素瘤细胞的免疫反应,作用机制可能与其抑制NK细胞或直接促进肿瘤细胞增殖有关,Nrp-1+T细胞可能是一种新的Treg亚群。OBJECTIVE：To verificate the immunoregulatory effect of neuropilin-1 positive T cells（Nrp-1＋T cells）.METHODS：A mouse B16-F10-luc-G5 melanoma in vivo model was established,and by using living image system,Nrp-1＋T cells sorted from mice splenic cells were injected into the model and its regulation on proliferation of B16-F10-luc-G5 melanoma cells was observed.RESULTS：The percentage of Nrp-1＋T cells in the mice splenic cells was（2.65±0.28）%.At the 6th day after modeling of B16-F10-luc-G5 melanoma,the detected photons number of the Nrp-1＋T cell group was higher than that of the non-Nrp-1＋T cell group,which were（3.69±0.04）×106 s-1 and（1.22±0.09）×105 s-1 respectively.The difference between two groups was significant（t=-43.438,P0.01）.CONCLUSIONS：Nrp-1＋T cells can suppress the immune activity aimed at B16-F10-luc-G5 melanoma in vivo.The mechanism may be concerned with Nrp-1＋T cells＇ suppression of NK cells or Nrp-1＋T cells＇ direct promotion effect on melanoma cells.Nrp-1＋T cells may be one new type of regulatory T cells.

关 键 词：黑色素瘤/免疫学 T淋巴细胞 细胞增殖 小鼠 

分 类 号：R730.2[医药卫生—肿瘤]