3.0T临床型MR多发性硬化大鼠脊髓成像的初步研究  

Imaging of the spinal cord in rat of multiple sclerosis: preliminary study by 3.0T clinical MR-scanner

作　　者：张海琴[1] 李坤成[2] 于春水[2] 秦文[2] 马佳[3] 

机构地区：[1]首都医科大学宣武医院核医学科,北京100053 [2]首都医科大学宣武医院放射科,北京100053 [3]首都医科大学宣武医院超声科,北京100053

出　　处：《医学影像学杂志》2010年第6期889-891,共3页Journal of Medical Imaging

基　　金：北京市自然科学基金(7042026);中外医学磁共振交流基金(N4-002)

摘　　要：目的：探索应用3.0T临床型MR设备进行多发性硬化（MS）大鼠脊髓成像的可行性。方法：用髓鞘少突胶质细胞糖蛋白多肽35-55（MOG35-55）致敏Lewis大鼠制备MS动物模型实验性自身免疫性脑脊髓炎（EAE）大鼠10只,正常对照组大鼠4只。3.0T临床型MR（Siemens Trio Tim）扫描仪配用正交腕关节线圈,行T2W、T1W和Gd-DTPA增强T1W的三维容积扫描,体素0.06-0.08mm3。结果：大鼠脊髓T2WI具有较高的空间分辨率,颈髓、胸髓和腰髓等节段显示清晰。脊髓灰质和白质的对比度较弱。MOG35-55-EAE大鼠病灶出现在颈髓和胸髓,矢状位重组图像显示病灶细节清晰,呈条片状,T2高信号,T1等信号,4个脊髓病灶均未见Gd-DTPA强化。结论：应用3.0T临床型MR扫描仪配备小正交腕关节线圈进行MOG35-55-EAE大鼠脊髓MR成像是可行的,为小动物脊髓疾病的活体体外监测提供了一种能广泛应用的无创性手段。Objective：To investigate the intravital visibility of spinal cord in rats of multiple sclerosis（MS） by using 3.0T clinical whole body magnetic resonance（MR）-scanners.Methods：Ten healthy Lewis rats were injected with the peptide 35-55 of myelin oligodendrocyte glycoprotein（MOG35-55） to become the experimental autoimmune encephalomyelitis（EAE）,the model of MS.Control group rats were four.On a Siemens Trio Tim 3.0 T MR-scanner equipped with a quadrature wrist coil,rats were examined using T2W,T1W and Gd-DTPA contrast T1W 3dimensional imaging with voxel size up to 0.06～0.08 mm^3.Results：T2W images of rat spinal cord with high spatial resolution could be obtained,but the contrast was less.The cervical spinal cord,thoracic spinal cord and lumbar spinal cord were clearly visible.The plaques in spinal cord of MOG35-55-EAE presented as hyperintense areas on T2WI and isointense areas on T1WI.Lesions were showed clearly on T2W sagittal reconstructed images.None of the 4 lesions showed enhancement on Gd-T1WI.Conclusion：3.0 T clinical MR-scanner can obtain high quality images of the rat spinal cord.It offers a noninvasive technique for studying central nervous system diseases in the rats.

关 键 词：大鼠 脊髓 多发性硬化 实验性自身免疫性脑脊髓炎 磁共振成像 

分 类 号：R744[医药卫生—神经病学与精神病学] R445.2[医药卫生—临床医学]