固定丝素蛋白膜中的联吡啶钌电化学发光行为的研究与应用  

Electrochemiluminescence Based on Silk Fibroin-Tris(2,2′-bipyridine)ruthenium(Ⅱ) Film Modified Glassy-carbon Electrode and Its Analytical Application

作　　者：李利军[1] 蔡卓[2] 蓝苏梅[2] 吴启涛[1] 程龙军[2] 郝学超[1] 

机构地区：[1]广西工学院生物与化学工程系,柳州545006 [2]广西大学化学化工学院,南宁530004

出　　处：《分析化学》2010年第6期778-782,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20665001);广西自然科学基金(No.0832062)资助项目

摘　　要：基于苯海拉明对联吡啶钌(Ru(bpy)32+)的电化学发光的增敏作用和丝素蛋白-联吡啶钌复合膜修饰玻碳电极稳定好的特点,建立了一种以丝素蛋白多孔膜-联吡啶钌复合物修饰的玻碳电极电化学发光检测苯海拉明的新方法。结果表明,该修饰电极具有很好的电化学活性和电化学发光(ECL)响应。在最佳实验条件下,苯海拉明浓度在1.0×10-4～1.0×10-7mol/L范围内与其相对发光强度呈良好的线性关系(r=0.9989);检出限为2.3×10-7mol/L(S/N=3)。连续平行测定3.78×10-5mol/L苯海拉明5次,发光强度的RSD为1.76%。用于实际样品中苯海拉明的测定,结果满意。Based on the increasing effect of diphenhydramine on Ru（bpy）32＋ electrochemiluminescence （ECL） system, an ECL method was developed for the determination of diphenhydramine hydrochloride by silk fibroin （SF）-Ru（bpy）2＋3 composite film modified glassy-carbon electrode. A highly-sensitive sensor was prepared based on SF-Ru（bpy）2＋3 composite film modified glass-carbon electrode. Its fabrication procedure, related experiment conditions and electrochemical characterization were studied. Under the optimum experimental condition, the linearity of diphenhydramine concentration to ECL intensity was achieved （r2=0.9989） in the concentration of diphenhydramine hydrochloride of 1.0×10-4-1.0×10-7 mol/L and the detection limit （S/N=3） was 2.3×10-7 mol/L. The RSD for 5 times determination of 3.78×10-5 mol/L diphenhydramine was 1.76%.

关 键 词：苯海拉明 电致化学发光 联吡啶钌 丝素蛋白 化学修饰电极 

分 类 号：R96[医药卫生—药理学]