MMP-1和TIMP-1在人增生性瘢痕中的动态变化  

Dynamic changes of matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metallpreteinase-1 (TIMP-1) in hypertrophic scar

作　　者：张鹏[1] 纪亮[1] 李静平[1] 沈雷[1] 陈永春[1] 李云峰[2] 

机构地区：[1]齐齐哈尔医学院解剖教研室,黑龙江齐齐哈尔161000 [2]齐齐哈尔医学院第三附属医院美容科,黑龙江齐齐哈尔161000

出　　处：《中国现代医学杂志》2010年第11期1665-1668,共4页China Journal of Modern Medicine

基　　金：黑龙江省自然科学基金课题项目(No:D200957)

摘　　要：目的通过比较MMP-1和TIMP-1在不同时期增生性瘢痕中的动态变化,探讨MMP-1和TIMP-1在增生性瘢痕发病过程中的作用。方法对13例中期增生性瘢痕和21例晚期增生性瘢痕手术切除标本采用免疫组织化学方法 ,进行定性定量研究。结果 MMP-1和TIMP-1在中期和晚期增生性瘢痕中均高表达,在普通瘢痕及正常皮肤中几乎不表达;MMP-1在中期增生性瘢痕表达水平要明显高于晚期增生性瘢痕,差异具有显著性(P<0.05);TIMP-1在晚期增生性瘢痕表达水平要明显高于中期增生性瘢痕,差异具有显著性(P<0.05)。结论中期增生性瘢痕组织增生旺盛可能与MMP-1表达增多有关;晚期增生性瘢痕重塑失败可能与此时TIMP-1的表达量增多有关。[Objective]To investigate the role of MMP-1 and TIMP-1 in the formation of hypertrophic scar （HS）, by comparing the dynamic changes of MMP-1 and TIMP-1 in different period of hypertrophic scar.[Methods]The tissues of hypertrophic scar were obtained from 34 patients, of which 13 cases with hypertrophic scar in middle period and 21 cases with hypertrophic scar in late period. The polyclone antibodies of anti-MMP-1 and anti-TIMP-1 were used to perform immunohistochemical staining （SABC method）. [Results]There was significant difference in the expression of MMP-1 and TIMP-1 between different period of hypertrophic scar groups and the two control groups. The levels of MMP-1 expression and TIMP-1 expression of hypertrophic scar in the two period were both significant higher than those in normal skin and normotrophic scar （P 0.05）. MMP-1 expression of hypertrophic scar in middle period was significant higher than that in the late period （P 0.05）, while TIMP-1 expression of hypertrophic scar in late period was significant higher than that in the middle period （P 0.05）.[Conclusion]MMP-1 may contribute to the hyperplasia of hypertrophic scar in middle period. The failure remodeling of hypertrophic scar in late period may correlate with TIMP-1.

关 键 词：增生性瘢痕 MMP-1 TIMP-1 

分 类 号：R62[医药卫生—整形外科]