多药耐药相关蛋白基因在乳腺癌中的表达及其临床意义  

Expression of Multidrug Resistance associated Protein Gene in Breast Cancer

作　　者：陈高明[1,2,3,4] 李春海[1,2,3,4] 毛慧生 王建军[1,2,3,4] 孙丽亚 王立梅[1,2,3,4] 杨毅[1,2,3,4] 王白岚 

机构地区：[1]军事医学科学院肿瘤分子生物学实验室 [2]解放军94医院 [3]济南市传染病医院 [4]天津市肿瘤研究所分子生物学实验室

出　　处：《中国肿瘤临床》1999年第1期23-25,共3页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金

摘　　要：目的：检测多药耐药相关蛋白（ＭＲＰ）基因在乳腺癌组织中的表达水平，并和谷胱甘肽Ｓ－转移酶－ｐｉ（ＧＳＴ－π）的表达作相关性比较，探讨其在临床的应用价值。方法：用定量ＲＴ－ＰＣＲ技术，检测了５５例乳腺癌组织中ＭＲＰ和其中４０例ＧＳＴ－πｍＲＮＡ的表达水平，并和术前化疗比较分析。结果：ＭＲＰ表达的阳性率为７６．４％，高表达占１８．２％，中低表达各占２３．６％和３４．５％。ＭＲＰ和ＧＳＴ－π有４０％的共表达率，虽然ＭＲＰ表达阳性率及水平和术前化疗关系不明显，但化疗后二者表达有正相关趋势。结论：ＭＲＰ在乳腺癌中普遍表达，以中低表达为主，其和ＧＳＴ－π的共表达可能和化疗药物诱导有关。Objective: The gene expression of multidrug resistance associated protein (MRP) in breast cancer and its relation with the glutathione S transferase π (GST π) were studied.Methods:The expression of MRP gene in 55 cases of breast cancer was measured by quantitative PCR, and the level of GST π was also determined in 40 cases.The correlation between chemotherapy and MRP expression was analysed as well. Results: Positive expression was shown in 76.4% of the cases. Of these, high level expression of MRP was found in 18.2% , median level in 23.6% and low level in 34.5%. Coexpression of MRP and GST π was seen in 40% of breast cancer. In addition,no relationship was found between the amount of MRP and effect of chemotherapy, but the level of MRP was higher than those who received chemotherapy before surgery.Conclusion:MRP is a major factor associated with drug resistance in breast cancer. MRP and GST might be induced by chemotherapeutic agents simultaneously.

关 键 词：MRP 乳腺癌 多药耐药性 基因表达 

分 类 号：R737.902[医药卫生—肿瘤]