尿苷三磷酸对大鼠局灶性缺血再灌注损伤脑组织的保护作用  

Protective effect of uridine 5’-triphosphate on brain tissue after cerebral ischemia-reperfusion injury in rats

作　　者：田谋利[1] 孙海静[1] 高欣[2] 宁慧杰[1] 石学银[1] 

机构地区：[1]第二军医大学长征医院麻醉科,上海200003 [2]第二军医大学长征医院影像科,上海200003

出　　处：《第二军医大学学报》2010年第6期608-611,共4页Academic Journal of Second Military Medical University

基　　金：上海市卫生局科研基金(2009125)~~

摘　　要：目的采用磁共振成像(MRI)法连续观察尿苷三磷酸(UTP)对大鼠局灶性脑缺血再灌注损伤的作用。方法线栓法制备大鼠中脑缺血再灌注(MCAO)损伤模型,大脑中动脉缺血30min后以微量输注泵采用大鼠尾静脉持续给予不同剂量的UTP,输注速度为5ml.kg-1.min-1。再灌注24h后测量大鼠脑含水量及神经损伤行为学评分(NDS),并采用TTC染色法观察大鼠脑梗死体积。采用MRI连续观察脑缺血再灌注后6、30、54h大鼠脑梗死情况。结果 10、30、90μg/kgUTP组大鼠NDS评分明显低于生理盐水对照组(P<0.01),大鼠右侧大脑半球含水量明显少于生理盐水对照组(P<0.01)。随着UTP剂量的增加,梗死区域逐渐缩小,10、30、90μg/kgUTP组梗死体积占整个脑半球体积的百分比均较生理盐水对照组明显减少(P<0.01)。与生理盐水对照组相比,90μg/kgUTP组再灌注后6、30、54h梗死区域明显减少,差异有统计学意义(P<0.01)。结论 UTP对大鼠局灶性缺血再灌注损伤脑组织具有保护作用,可显著减少脑梗死体积。Objective To use MRI method for continuous observation of the protective effect of uridine 5’-triphosphate （UTP） against cerebral ischemia-reperfusion （IR） injury in rats.Methods The cerebral ischemia was induced by middle cerebral artery occlusion （MCAO） in rats.UTP solution was delivered through an indwelling tail venous catheter via microinfusion pump 30 min after MCAO at a rate of 5 ml·kg-1·min-1.Neurological deficit score （NDS） and brain water content were determined 24 h after reperfusion.The infarction volume was determined by 2,3,5-triphenyl-tetrazolium chloride （TTC） staining and MRI at 6,30,and 54 h after reperfusion.Results The NDS scores in 10,30,and 90 μg/kg UTP groups were significantly lower than that in the saline control group（P0.01）;the water contents in the right cerebral hemisphere of 10,30,and 90 μg/kg UTP groups were significantly less than that in the normal saline group（P0.01）.The cerebral infarction volume was gradually decreased with the increase of UTP dose,and the proportions of infarction volumes in 10,30,and 90 μg/kg UTP groups were significantly decreased compared with the saline control group（P0.01）.The infarction volumes at 6,30,and 54 h after reperfusion in 90 μg/kg UTP group were significantly lower than those in the saline control group（P0.01）.Conclusion UTP has a protective effect on focal cerebral ischemia-reperfusion injury in rats;it can greatly reduce the volume of cerebral infarction.

关 键 词：尿苷三磷酸 再灌注损伤 脑缺血 脑梗死 

分 类 号：R743.3[医药卫生—神经病学与精神病学]