FHIT和MDM2在口腔黏膜下纤维性变及其癌变组织中的表达  

Expression of FHIT and MDM2 in oral submucous fibrosis and canceration tissues

作　　者：尹晓敏[1] 温春燕[2] 韩玉玲[2] 高义军[2] 唐瞻贵[3] 

机构地区：[1]中南大学湘雅医院口腔内科,长沙410078 [2]中南大学湘雅二医院口腔科,长沙410011 [3]中南大学湘雅医院口腔颌面外科,长沙410078

出　　处：《中南大学学报（医学版）》2010年第6期572-575,共4页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(30872895);湖南省卫生厅科研基金(B2007-050)~~

摘　　要：目的:初步探讨脆性组氨酸三联体基因(fragile histidine triad,FHIT)、原癌基因MDM2在口腔黏膜下纤维性变(oral submucous fibrosis,OSF)和癌变过程中的作用。方法:采用免疫组织化学SP法检测44例OSF组织、15例OSF癌变组织及10例正常口腔黏膜组织中FHIT和MDM2蛋白的表达及分布。结果:FHIT在正常口腔黏膜中强阳性表达,在OSF及OSF癌变组织中阳性表达率逐渐减少,OSF组及OSF癌变组织组显著低于正常对照组(P<0.05);OSF癌变组织组显著低于OSF组(P<0.05)。MDM2蛋白在正常口腔黏膜中呈阴性表达,在OSF及OSF癌变组织中阳性表达率逐渐增加,OSF组及OSF癌变组织组显著高于正常对照组(P<0.05),OSF癌变组织组显著高于OSF组(P<0.05)。结论:FHIT表达下调和缺失,以及MDM2过度表达可能在OSF癌变过程中具有重要作用。Objective To determine the role of fragile histidine triad （FHIT） and MDM2 in carcinogenesis of oral submucous fibrosis（OSF）.Methods The expression of FHIT and MDM2 was examined by immunohistochemical S-P method in 44 OSF cases,15 canceration tissues of OSF,and 10 normal oral mucosa tissues.Results The expression of FHIT was positive in the normal oral mucosa epithelium.The positive expression of FHIT decreased in the OSF and canceration tissues of the OSF.The rate of FHIT positive expression was significantly lower in canceration tissues of OSF than that of the OSF（P0.05）.The expression of MDM2 was negative in normal oral mucosa epithelium.The positive expression of MDM2 increased in the OSF and canceration tissues of the OSF,and the rate of MDM2 positive expression was significantly higher in the canceration tissues of OSF than that of the OSF（P0.05）.Conclusion The loss of FHIT and over-expression of MDM2 may play an important role in the carcinogenesis of OSF.

关 键 词：口腔黏膜下纤维性变 癌变 脆性组氨酸三联体基因 MDM2 免疫组织化学 

分 类 号：R739.8[医药卫生—肿瘤]