全反式维甲酸对人胃腺癌细胞中Akt及其磷酸化蛋白表达的影响  

Effects of all trans - retinoic acid on the expession of Akt and p - Akts in human gastric carcinoma cell line

作　　者：杨艳梅[1] 张春艳[1] 马玉彦[1] 杨悦[1] 任丽君[1] 秦莉[1] 袁玉涛[1] 

机构地区：[1]哈尔滨医科大学肿瘤研究所,哈尔滨150081

出　　处：《实用肿瘤学杂志》2010年第3期209-211,223,共4页Practical Oncology Journal

基　　金：黑龙江省教育厅资助课题（11521182）

摘　　要：目的研究全反式维甲酸（alltrans-retinoic acid，ATRA）对人胃腺癌细胞（SGC-7901）中Akt及其磷酸化蛋白p—Akt（Ser473）和p—Akt（Thr308）表达的影响及其对细胞凋亡的诱导作用。方法采用四甲基偶氮唑盐（MTT）方法检测ATRA对SGC-7901增殖的抑制作用；荧光混微镜观察细胞凋亡形态；Western blot方法检测不同浓度ATRA处理后的SGC-7901细胞中Akt、p-Akt（Ser473）和p-Akt（Thr308）的表达情况。结果ATRA对SGC-7901细胞增殖的抑制作用呈时间和剂量依赖性；ATRA处理48h时，荧光显微镜观察细胞呈现明显的凋亡形态学改变；Western blot检测显示ATRA对细胞中Akt蛋白的表达没有明显影响，但显著下调两种p-Akt蛋白的表达。结论ATRA诱导SGC～7901细胞凋亡可能与其抑制磷酸化Akt蛋白表达有关。Objective To investigate the effeets of all trans -retinoic acid（ ATRA） on the expression of Akt or its phosphorylated proteins and on apoptosis of human gastric carcinoma cell line（ SGC -7901 ）. Methods The effects of ATRA on the cell proliferation was measured by methyl thiazolyl tetrazolium（MTT） ,the apoptosis of ATRA on SGC- 7901cells was observed by fluorescentmieroscope, and the expression of Akt, p- Akt （ Ser473 ） and p - Akt （ Thr308 ） were measured by Western blot. Results ATRA inhibits the proliferation of SGC -7901 cells in a dose and time dependent manner. SGC -7901 cells showing typical apoptosis morphological changes were induced by ATRA at 48 h. ATRA did not influence the expression of Akt, but significantly decreased the expression of p - Akt （ Ser473 ） and p - Akt（ Thr308 ）. Conclution ATRA could induce apoptosis by down - regulating expression of p - Akt（ Ser473 ） and p - Akt（ Thr308 ）.

关 键 词：全反式维甲酸 胃癌 AKT 

分 类 号：R735.1[医药卫生—肿瘤]